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dc.contributor.authorValenzuela, Jacob J.
dc.contributor.authorCarlson, Ross P.
dc.contributor.authorGerlach, Robin
dc.contributor.authorCooksey, Keith E.
dc.contributor.authorPeyton, Brent M.
dc.contributor.authorBothner, Brian
dc.contributor.authorFields, Matthew W.
dc.date.accessioned2016-03-30T17:41:58Z
dc.date.available2016-03-30T17:41:58Z
dc.date.issued2013-08
dc.identifier.citationValenzuela, J., R. P. Carlson, R. Gerlach, K. Cooksey, B. M. Peyton, B. Bothner, and M. W. Fields. “Nutrient Resupplementation Arrests Bio-Oil Accumulation in Phaeodactylum Tricornutum.” Applied Microbiology Biotechnology 97, no. 15 (June 15, 2013): 7049–7059. doi:10.1007/s00253-013-5010-y.en_US
dc.identifier.issn0175-7598
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9651
dc.description.abstractPhaeodactylum tricornutum is a marine diatom in the class Bacillariophyceae and is important ecologically and industrially with regards to ocean primary production and lipid accumulation for biofuel production, respectively. Triacylglyceride (TAG) accumulation has been reported in P. tricornutum under different nutrient stresses, and our results show that lipid accumulation can occur with nitrate or phosphate depletion. However, greater lipid accumulation was observed when both nutrients were depleted as observed using a Nile Red assay and fatty acid methyl ester (FAME) profiles. Nitrate depletion had a greater effect on lipid accumulation than phosphate depletion. Lipid accumulation in P. tricornutum was arrested upon resupplementation with the depleted nutrient. Cells depleted of nitrogen showed a distinct shift from a lipid accumulation mode to cellular growth post-resupplementation with nitrate, as observed through increased cell numbers and consumption of accumulated lipid. Phosphate depletion caused lipid accumulation that was arrested upon phosphate resupplementation. The cessation of lipid accumulation was followed by lipid consumption without an increase in cell numbers. Cells depleted in both nitrate and phosphate displayed cell growth upon the addition of both nitrate and phosphate and had the largest observed lipid consumption upon resupplementation. These results indicate that phosphate resupplementation can shut down lipid accumulation but does not cause cells to shift into cellular growth, unlike nitrate resupplementation. These data suggest that nutrient resupplementation will arrest lipid accumulation and that switching between cellular growth and lipid accumulation can be regulated upon the availability of nitrogen and phosphorus.en_US
dc.description.sponsorshipSupport was provided by the Air Force Office of Scientific Research (DOD-AFOSR grant FA9550-09-1-0243), the Molecular Bioscience Program at Montana State University, and NSF-EPSCoR. We are also thankful for laboratory contributions by Erika Whitney. We would also like to thank the Murdock Charitable Trust and NIH Cobre 5P20RR02437-03 for support of the mass spectrometry facility at MSU. This material is also based upon work supported by the National Science Foundation under CHE-1230632.en_US
dc.titleNutrient resupplementation arrests bio-oil accumulation in Phaeodactylum tricornutumen_US
dc.typeArticleen_US
mus.citation.extentfirstpage7049en_US
mus.citation.extentlastpage7059en_US
mus.citation.issue15en_US
mus.citation.journaltitleApplied Microbiology and Biotechnologyen_US
mus.citation.volume97en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/s00253-013-5010-yen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage7en_US


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