Show simple item record

dc.contributor.advisorChairperson, Graduate Committee: Aleksander Rebaneen
dc.contributor.authorStoltzfus, Caleb Rayen
dc.date.accessioned2016-10-23T21:27:08Z
dc.date.available2016-10-23T21:27:08Z
dc.date.issued2016en
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9861en
dc.description.abstractTwo-photon excited fluorescence (2PEF) is a unique photophysical process that has benefited many diverse areas of science. Imaging the 2PEF signal offers numerous intrinsic benefits, including low background scattering, high sample photo-stability, and high excitation selectivity. The 2PEF signal has a nonlinear dependence on excitation intensity, which has proven to be extremely useful for high resolution, three dimensional microscopy. This same nonlinear dependence, in conjunction with the typically low probability of two-photons being simultaneously absorbed, also makes 2PEF imaging difficult to scale, leaving most two-photon microscopes with a field of view (FOV) limited to less than a few mm 2. This effectively limits the benefits of the unique properties of 2PEF imaging to microscopic applications. This dissertation explores the development and application of a wide FOV 2PEF imaging technique, where a FOV as large as 10 cm 2 is achieved by increasing the peak photon flux of the excitation source, and expanding the illumination region. The use of this imaging technique for the in depth characterization and optimization of fluorescent proteins (FPs), as well as taking high contrast images of fingermarks is described. This new wide FOV 2PEF imaging technique greatly expands the usefulness of the unique photophysical properties of 2PEF and allows for sensitive, high contrast 2PEF imaging on a much larger scale than was previously possible.en
dc.language.isoenen
dc.publisherMontana State University - Bozeman, College of Letters & Scienceen
dc.subject.lcshMultiphoton excitation microscopyen
dc.subject.lcshFingerprintsen
dc.subject.lcshCyanoacrylatesen
dc.titleWide field of view two-photon excited fluorescence imaging, theory and applicationsen
dc.typeDissertationen
dc.rights.holderCopyright 2016 by Caleb Ray Stoltzfusen
thesis.catalog.ckey3149359en
thesis.degree.committeemembersMembers, Graduate Committee: Wm. Randall Babbitt; John L. Carlsten; Kevin S. Repasky; Bern Kohleren
thesis.degree.departmentPhysics.en
thesis.degree.genreDissertationen
thesis.degree.namePhDen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage140en
mus.data.thumbpage156en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record


MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.