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dc.contributor.authorHarrold, Zöe R.
dc.contributor.authorSkidmore, Mark L.
dc.contributor.authorHamilton, Trinity L.
dc.contributor.authorDesch, Elizabeth
dc.contributor.authorKirina, Amada
dc.contributor.authorvan Gelder, Will
dc.contributor.authorGlover, Kevin
dc.contributor.authorRoden, Eric E.
dc.contributor.authorBoyd, Eric S.
dc.date.accessioned2016-08-02T20:44:28Z
dc.date.available2016-08-02T20:44:28Z
dc.date.issued2015-12
dc.identifier.citationHarrold, Zöe R. , Mark L. Skidmore, Trinity L. Hamilton, Elizabeth Desch, Amada Kirina, Will van Gelder, Kevin Glover, Eric E. Roden, and Eric S. Boyd. "Aerobic and Anaerobic Thiosulfate Oxidation by a Cold-Adapted, Subglacial Chemoautotroph." Applied and Environmental Microbiology 82, no. 5 (December 2015 ): 1486-1495. DOI: 10.1128/AEM.03398-15.en_US
dc.identifier.issnApplied and Environmental Microbiology
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9961
dc.description.abstractGeochemical data indicate that protons released during pyrite (FeS2) oxidation are important drivers of mineral weathering in oxic and anoxic zones of many aquatic environments including those beneath glaciers. Oxidation of FeS2 under oxic, circumneutral conditions proceeds through the metastable intermediate thiosulfate (S2O32-), which represents an electron donor capable of supporting microbial metabolism. Subglacial meltwaters sampled from Robertson Glacier (RG), Canada over a seasonal melt cycle reveal concentrations of S2O32- that are typically below detection despite the presence of available pyrite and several orders of magnitude higher concentrations of the FeS2 oxidation product sulfate (SO42-). Here we report the physiological and genomic characterization of the chemolithoautotrophic facultative anaerobe Thiobacillus sp. RG5 isolated from the subglacial environment at RG. The RG5 genome encodes pathways for the complete oxidation of S2O32-, CO2 fixation, and aerobic and anaerobic respiration with nitrite or nitrate. Growth experiments indicate that the energy required to synthesize a cell under oxygen or nitrate reducing conditions with S2O32- as electron donor was lower at 5.1 °C than 14.4 °C, indicating that this organism is cold-adapted. RG sediment-associated soxB transcripts, which encode a component of the S2O32--oxidizing complex, were closely affiliated to soxB from RG5. Collectively, these results suggest an active sulfur cycle in the subglacial environment at RG mediated in part by populations closely affiliated with RG5. Microbial consumption of S2O32- by RG5-like populations may accelerate abiotic FeS2 oxidation thereby enhancing mineral weathering in the subglacial environment.en_US
dc.description.sponsorshipNASA grants NNX10AT31G (MLS and ESB) and NNA15BB02A527 (ESB)en_US
dc.titleAerobic and Anaerobic Thiosulfate Oxidation by a Cold-Adapted, Subglacial Chemoautotrophen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1486en_US
mus.citation.extentlastpage1495en_US
mus.citation.issue5en_US
mus.citation.journaltitleApplied and Environmental Microbiologyen_US
mus.citation.volume82en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1128/AEM.03398-15en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentEarth Sciences.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupThermal Biology Institute.en_US
mus.data.thumbpage9en_US


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