Show simple item record

dc.contributor.authorDepres, Jordane
dc.contributor.authorForano, Evelyne
dc.contributor.authorLepercq, Pascale
dc.contributor.authorComtet-Marre, Sophie
dc.contributor.authorJubelin, Grégory
dc.contributor.authorYeoman, Carl J.
dc.contributor.authorBerg Miller, Margret E.
dc.contributor.authorFields, Christopher J.
dc.contributor.authorTerrapon, Nicolas
dc.contributor.authorLe Bourvellec, Carine
dc.contributor.authorRenard, Catherine M.G.C.
dc.contributor.authorHenrissat, Bernard
dc.contributor.authorWhite, Bryan A.
dc.contributor.authorMosoni, Pascale
dc.date.accessioned2016-08-18T15:20:40Z
dc.date.available2016-08-18T15:20:40Z
dc.date.issued2016-02
dc.identifier.citationDespres, Jordane , Evelyne Forano, Pascale Lepercq, Sophie Comtet-Marre, Grégory Jubelin, Carl J. Yeoman, Margret E. Berg Miller, Christopher J. Fields, Nicolas Terrapon, Carine Le Bourvellec, Catherine M.G.C. Renard, Bernard Henrissat, Bryan A. White, and Pascale Mosoni. "Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis." BMC Genomics 17, no. 1 (February 2016): 147. DOI: 10.1186/s12864-016-2472-1.en_US
dc.identifier.issn1471-2164
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9990
dc.description.abstractBackground: Diet and particularly dietary fibres have an impact on the gut microbiome and play an important role in human health and disease. Pectin is a highly consumed dietary fibre found in fruits and vegetables and is also a widely used additive in the food industry. Yet there is no information on the effect of pectin on the human gut microbiome. Likewise, little is known on gut pectinolytic bacteria and their enzyme systems. This study was undertaken to investigate the mechanisms of pectin degradation by the prominent human gut symbiont Bacteroides xylanisolvens. Results: Transcriptomic analyses of B. xylanisolvens XB1A grown on citrus and apple pectins at mid- and late-log phases highlighted six polysaccharide utilization loci (PUL) that were overexpressed on pectin relative to glucose. The PUL numbers used in this report are those given by Terrapon et al. (Bioinformatics 31(5):647-55, 2015) and found in the PUL database: http://www.cazy.org/PULDB/. Based on their CAZyme composition, we propose that PUL 49 and 50, the most overexpressed PULs on both pectins and at both growth phases, are involved in homogalacturonan (HG) and type I rhamnogalacturonan (RGI) degradation, respectively. PUL 13 and PUL 2 could be involved in the degradation of arabinose-containing side chains and of type II rhamnogalacturonan (RGII), respectively. Considering that HG is the most abundant moiety (>70 %) within pectin, the importance of PUL 49 was further investigated by insertion mutagenesis into the susC-like gene. The insertion blocked transcription of the susC-like and the two downstream genes (susD-like/FnIII). The mutant showed strong growth reduction, thus confirming that PUL 49 plays a major role in pectin degradation. Conclusion: This study shows the existence of six PULs devoted to pectin degradation by B. xylanisolvens, one of them being particularly important in this function. Hence, this species deploys a very complex enzymatic machinery that probably reflects the structural complexity of pectin. Our findings also highlight the metabolic plasticity of B. xylanisolvens towards dietary fibres that contributes to its competitive fitness within the human gut ecosystem. Wider functional and ecological studies are needed to understand how dietary fibers and especially plant cell wall polysaccharides drive the composition and metabolism of the fibrolytic and non-fibrolytic community within the gut microbial ecosystem.en_US
dc.description.sponsorshipEuropean Union’s Seventh Framework Program (FP/2007/2013)/European Research Council (ERC) Grant Agreement 322820
dc.titleUnraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesisen_US
dc.typeArticleen_US
mus.citation.extentfirstpage147en_US
mus.citation.issue1en_US
mus.citation.journaltitleBMC Genomicsen_US
mus.citation.volume17en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1186/s12864-016-2472-1en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentAnimal & Range Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.