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dc.contributor.authorCui, Jun
dc.contributor.authorRotstein, Megan
dc.contributor.authorBennett, Theo
dc.contributor.authorZhangm, Pengbo
dc.contributor.authorXia, Ninuo
dc.contributor.authorReijo Pera, Renee A.
dc.identifier.citationCui, Jun, Megan Rotstein, Theo Bennett, Pengbo Zhangm, Ninuo Xia, and Renee A. Reijo Pera. "Quantification of dopaminergic neuron differentiation and neurotoxicity via a genetic reporter.." Scientific Reports 6 (April 2016): 25181 . DOI: 10.1038/srep25181.en_US
dc.description.abstractHuman pluripotent stem cells provide a powerful human-genome based system for modeling human diseases in vitro and for potentially identifying novel treatments. Directed differentiation of pluripotent stem cells produces many specific cell types including dopaminergic neurons. Here, we generated a genetic reporter assay in pluripotent stem cells using newly-developed genome editing technologies in order to monitor differentiation efficiency and compare dopaminergic neuron survival under different conditions. We show that insertion of a luciferase reporter gene into the endogenous tyrosine hydroxylase (TH) locus enables rapid and easy quantification of dopaminergic neurons in cell culture throughout the entire differentiation process. Moreover, we demonstrate that the cellular assay is effective in assessing neuron response to different cytotoxic chemicals and is able to be scaled for high throughput applications. These results suggest that stem cell-derived terminal cell types can provide an alternative to traditional immortal cell lines or primary cells as a quantitative cellular model for toxin evaluation and drug discovery.en_US
dc.description.sponsorshipU01 HL100397 (project 2)en_US
dc.titleQuantification of dopaminergic neuron differentiation and neurotoxicity via a genetic reporteren_US
mus.citation.journaltitleScientific Reportsen_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentMicrobiology & Cell Biology.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.contributor.orcidReijo Pera, Renee A.|0000-0002-6487-1329en_US

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