Theses and Dissertations at Montana State University (MSU)
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/733
Browse
12 results
Search Results
Item Impact of cattle feeding-style on beef and human postprandial inflammation(Montana State University - Bozeman, College of Education, Health & Human Development, 2024) Spears, Meghan Leigh; Chairperson, Graduate Committee: Mary P. Miles; This is a manuscript style paper that includes co-authored chapters.Purpose: Various cattle-feeding styles have arisen in recent years, impacting the sustainability and environmental practices of many producers. While these changes are known to have an impact on the environment, little is known about the direct impact cattle raised using different feeding styles has on human health. Acute response focused studies, like this, are a glimpse into the expected impact of a certain food on the diet over time. The purpose of this study is to investigate the impact of cattle-feeding style on postprandial inflammation. Methods: A randomized, double blind, crossover study design was used to compare grass-fed (GRA) and conventional (CON) beef. Subjects (n=10) were comprised of men and women with a healthy body mass index (BMI) and no preexisting metabolic conditions. Blood samples were collected fasted and postprandially for four hours. Blood samples were analyzed for inflammation markers (TNF-alpha, IL-23, IL-17, IL-10, IL-1beta, IL-6, IFN-gamma and GM-CSF) at hourly timepoints. To observe postprandial changes with and without consideration for cattle feeding style, the net area under the curve (AUC) was calculated. Maxchange and CMAXtime were calculated by finding the maximum value of each cytokine between hours one and hour postprandially and subtracting that from the fasting value. CMAXtime represents the time at which the maximum value of each cytokine was reached in hours. Maxchange and AUC responses were compared to zero using a one-sample t-test to determine if response was greater than fasting. Results: In response to beef, maxchange of all measured markers and IFNgamma AUC were significantly greater than zero (p< 0.05). No differences were shown between GRA and CON in inflammation AUC (p> 0.05). Conclusion: This demonstrates that beef consumption does increase postprandial inflammation, but cattle-feeding style does not significantly impact this response.Item Validating Salmonella typhimurium virulence modulation by ecto-5'-nucleotidase (CD73) in intestinal epithelial cells(Montana State University - Bozeman, College of Letters & Science, 2017) Schneider, Stephanie Lorain; Chairperson, Graduate Committee: Douglas KominskyExtracellular ATP is a pro-inflammatory molecule released during intestinal insult and must be converted to adenosine by ecto-5'-nucleotidase (CD73) for the resolution of intestinal inflammation [1]. Along with its anti-inflammatory role in the intestinal mucosa, CD73-generated adenosine contributes to host-microbe interactions at the mucosal surface by modulating pathogen replication and virulence, including that of Salmonella enterica serovar Typhimurium (S. Typhimurium) [2], [3]. It has been shown, in the absence of intestinal epithelial cell-specific CD73, S. Typhimurium virulence is attenuated in vivo and in vitro, implicating the intestinal epithelium as an underappreciated source for the development of novel antimicrobial therapies. Since direct modulation of extracellular adenosine leads to pleiotropic effects, the aim of this research was to determine the mechanism(s) of S. Typhimurium virulence modulation by CD73 in intestinal epithelial cells to identify specific molecular targets that modulate pathogenesis [4], [5]Item Post-prandial serum concentrations of Trimethylamine-N-oxide in overweight and obese adults with low and high systemic inflammation(Montana State University - Bozeman, College of Education, Health & Human Development, 2018) Wilson, Stephanie Michelle; Chairperson, Graduate Committee: Mary P. MilesPurpose: Elevated trimethylamine-N-oxide (TMAO) levels have been implicated in the development of atherosclerosis. The impact of a high-fat meal on the post-prandial TMAO response in healthy overweight and obese adults was explored, accounting for additional variables that may affect the rate of TMAO conversion. Methods: Forty overweight and obese men and women participated in a high-fat meal challenge containing 50 g fat. Blood samples were collected at fasting and hourly in the 4 hours after meal ingestion and tested for concentrations of TMAO, liver enzymes, lipids, creatinine, insulin, and inflammatory markers. An initial mixed-effects model was constructed to analyze TMAO changes in participants who classified as having low or high levels of systemic inflammation accounting for factors that may influence TMAO concentrations. Backward refinement of the initial model was performed based off p-values. Results: Model refinement found that the log TMAO response was best explained by inclusion of estimated glomerular filtration rate (eGFR), insulin over time, a linear interaction between time and inflammation phenotype, and random effects on subject. Log TMAO after the meal was not different between inflammation phenotypes (p = 0.33). Estimated GFR was the most important determinant in the log TMAO response (p = 0.03), with lower log TMAO observed with higher eGFR values. In conclusion, serum TMAO concentrations during a high fat-meal challenge were strongly determined by eGFR, but not by fasting inflammation status.Item Inflammatory effects of food available through the food distribution program on Indian reservations (FDPIR; commodities)(Montana State University - Bozeman, College of Education, Health & Human Development, 2018) Smith, Melanie Sue; Chairperson, Graduate Committee: Mary P. Miles; Mary P. Miles, Elizabeth Rink and Suzanne Held were co-authors of the article, 'Inflammatory effects of food available through the food distribution program on Indian reservations (FDPIR; commodities)' which is contained within this thesis.American Indian and Alaskan Native (AI/AN) populations experience some of the greatest health disparities and the lowest life expectancy in the United States compared to all other races and ethnicities. The Food Distribution Program on Indian Reservations (FDPIR) is a primary food supplement program that serves AI/AN communities in the United States. Recent studies have reported that FDPIR monthly food packages do not meet Dietary Guidelines for Americans (DGA). This study measured the effects of two FDPIR diets on inflammation, appetite, and energy intake to better understand potential health outcomes of each FDPIR diet. A within-subjects, randomized, crossover design was used to compare two dietary conditions: 1) FDPIR diet that met Dietary Guidelines for Americans (DGA); and 2) a typical FDPIR diet. Participants were AI/AN and non-AI/AN men and women (n=13), 18-55 years of age, with a waist circumference of > 94 cm for men and > 80 cm for women. Salivary interleukin 1 beta (IL-1beta) and interleukin 6 (IL-6) were collected at six separate time points over 24 hours to determine inflammatory response for each condition. Self-reported visual analogue scale (VAS) appetite questionnaires were used to gauge the effect of each condition on specific appetite sensations (hunger, fullness, satiety, desire to eat, and prospective consumption). Daily energy intake was calculated by weighing food in grams before and after each test day. There were no significant differences in inflammatory response and appetite sensations between the two dietary conditions found by RMANOVA (p<0.05). Participants ate 14% more (p<0.01) kilocalories on a typical FDPIR diet compared to FDPIR diet that met DGA. Higher energy intakes during a typical FDPIR diet compared to a FDPIR diet that meets DGA may increase risk for obesity and nutrition-related diseases, including type 2 diabetes, cardiovascular disease and other chronic inflammatory conditions. This project was approved by Montana State University Institutional Review Board (IRB) and is supported by National Institute of General Medical Sciences of the National Institutes of Health award number P20GM103474.Item First and second generational effects of colchicine on C57 black mice(Montana State University - Bozeman, College of Agriculture, 1980) Greathouse, Janice KayItem Demonstration of the leukocyte-endothelial cell adhesion cascade involved in lymphocyte recirculation and neutrophil and lymphocyte localization to sites of inflammation(Montana State University - Bozeman, College of Agriculture, 1994) Bargatze, Robert FrostItem The effects of carbohydrate on inflammation following an acute bout of resistance exercise(Montana State University - Bozeman, College of Education, Health & Human Development, 2006) Pearson, Sherri Diane; Chairperson, Graduate Committee: Mary P. Miles.The immune response to inflammation involves the release of cytokines, which determine the intensity and duration of the immune response (Kuby, 1997). The cytokine interleukin-6 (IL-6), functions as a negative feedback signal that turns off pro-inflammatory mediators during the immune response. IL-6 also initiates the release of CRP, which induces inflammation. Therefore, IL-6 is known as both a pro and anti-inflammatory mediator of the immune response. IL-6 is released during the immune response to inflammation. IL-6 peaks about 8 hours after an eccentric exercise session that induces muscle damage. Carbohydrate ingestion during endurance exercise attenuates the rise in IL-6 immediately post-exercise during recovery. IL-6 along with the acute phase protein C-reactive protein (CRP) (a marker of the systemic inflammatory response), and creatine kinase (CK) (a semi-quantitative marker of muscle damage), will be used to determine the affects of eccentric exercise on muscle damage and the inflammatory response.Item Inflammatory response to a high-force eccentric exercise protocol in oral contraceptive users and non-users(Montana State University - Bozeman, College of Education, Health & Human Development, 2008) Kasper, Christine, 1959-; Chairperson, Graduate Committee: Mary P. Miles.Researchers have demonstrated that oral contraceptive users have elevated serum C-reactive protein (CRP) levels. However, it is unclear whether or not CRP is elevated in response to inflammation. Results of preliminary studies indicate that individuals with elevated CRP exhibit diminished release of interleukin-6 (IL-6), an inflammatory mediator with anti-inflammatory properties. Low IL-6 may cause less control of inflammation and a resultant elevation of CRP. The inflammatory response was compared between female oral contraceptive (OC) users and non-OC users because of the known elevation of CRP in OC users. A high-force eccentric exercise protocol was used to induce muscle damage to the biceps brachii via 45 repetitions of the elbow flexors of the non-dominant limb. Blood was collected pre-exercise and at 4-, 8-, 12-, 24-, 48-, 96-, and 120-h post-exercise for analysis of muscle damage and inflammatory markers.Item Do carbohydrates increase the magnitude of the inflammatory response(Montana State University - Bozeman, College of Education, Health & Human Development, 2008) Depner, Chris M.; Chairperson, Graduate Committee: Mary P. Miles.Inflammation is the body's response to tissue damage and infection and is correlated with several chronic diseases like type II diabetes. Cytokines are cell signaling proteins with multiple functions including control of inflammation. Cytokines are influenced by several factors such as carbohydrate intake and exercise. Thus, carbohydrate intake and exercise can influence inflammation. Purpose: To determine the influence of high carbohydrate intake on the inflammatory response to exercise that induces muscle damage and inflammation. Methods: The study was a cross-over design. Each subject completed a high carbohydrate condition and a high fat and protein condition. Each condition consisted of 6 sets of 10 maximal high-force eccentric contractions of the elbow flexors and extensors. The exercise was followed by a controlled diet for the first 8 hours post-exercise based on the condition. The cytokines interleukin-6 (IL-6) and interleukin-1B (IL-B) were measured as indicators of local inflammation. C-reactive protein (CRP) was measured as an indicator of systemic inflammation. Creatine-kinase (CK), muscle soreness, upper arm circumference, and strength loss were measured as indicators of muscle damage. Blood glucose and insulin were measured to identify differences between diets in the conditions. Results: Insulin was significantly increased in the high carbohydrate condition compared to the high fat and protein condition at 1.5, 4, and 8 hours post-exercise. Perceived soreness was elevated at all time points post-exercise in both conditions and was significantly elevated in the high carbohydrate condition compared to the high fat and protein condition. There was a main effect trend for IL-6 to be greater in the high carbohydrate condition compared to the high fat and protein condition. Il-1B was significantly increased 24 hours post-exercise in the high carbohydrate condition compared to the high fat and protein condition. Conclusion: Elevated carbohydrate intake post-exercise augmented the local inflammatory response to the exercise observed by elevated IL-1B and IL-6. The augmented inflammatory response contributed to greater perceived muscle soreness post-exercise. Further research is required to investigate this mechanism further to provide better prevention and treatment methods for chronic diseases related to inflammation.Item Clinical implications of indomethacin superfused over the capillaries of frogs with activated white blood cells(Montana State University - Bozeman, College of Nursing, 2010) Priebe, Milissa Ann; Chairperson, Graduate Committee: Elizabeth S. KinionTreatment for inflammation is controversial. The purpose of this study was to investigate the effect of indomethacin on capillary permeability in animals with signs of systemic inflammation. We hypothesized that the permeability of an individual capillary would be lower after a shear stress challenge in the presence of indomethacin when evidence of systemic infection was present in the animal. Frogs (n=13) were pithed and the mesentery was exposed, hydraulic conductivity (L p) was assessed at 30 cm H 2O using the modified Landis technique after an abrupt change in shear stress. Two capillaries from each frog were used; one was a control and one with indomethacin superfused over the tissue. The frogs showed a systemic infection (nitro blue tetrazolium activation) but individual capillaries had no evidence of rolling or sticking white cells. There was a significant decrease in L p (P=0.002) when comparing the control and treatment vessels. The results of the analysis indicate capillary L p assessed in mesentery of infected frogs, decreased when exposed to shear stress and indomethacin. The data imply that gaps between endothelial cells may get smaller when indomethacin is introduced into the system decreasing the flow of fluids out of the capillary.