College of Agriculture

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As the foundation of the land grant mission at Montana State University, the College of Agriculture and the Montana Agricultural Experiment Station provide instruction in traditional and innovative degree programs and conduct research on old and new challenges for Montana’s agricultural community. This integration creates opportunities for students and faculty to excel through hands-on learning, to serve through campus and community engagement, to explore unique solutions to distinct and interesting questions and to connect Montanans with the global community through research discoveries and outreach.

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Now showing 1 - 6 of 6
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    Protein Supplementation and 48-Hour Calf Removal Effects on Range Cows
    (1992-03) Sowell, Bok F.; Wallace, Joe D.; Parker, Eugene E.; Southward, Morris G.
    In 1984, 99 Angus × Hereford cows (4- to 6-yr-olds) were assigned randomly to a 4-yr, 2 × 2 factorial study. Treatment assignment was permanent, and no new cows were added during the study. By 1987, 71 cows remained, and over-all, 335 complete cow-calf data sets were used. Main effect treatments were beginning time (prepartum [PRE] vs postpartum [POST]) for crude protein (CP) supplementation (twice weekly feeding of 41% CP cottonseed meal pellets at 1.58 kg • $\text{cow}^{-1}$ • $\text{feeding}^{-1}$) and temporary calf removal (48 hour [48-H] vs 0 hour [CONT]) just before the breeding season. For analyses, sex of calf was included as a third main effect (2×2×2) and year was included as a random factor; the 4-way interaction served as the testing term for repeated measures over years. Year was the dominant source of variation for most traits; we attributed this mainly to different amounts and timing of precipitation among years. Very few interactions were observed. The PRE supplemented cows had reduced (P<0.01) spring body weight losses and higher prebreeding body condition scores (4.9 vs 4.5; P<0.01) compared with POST cows. Reproductive performance did not differ between PRE and POST cows. Use of 48-H calf removal vs CONT did not influence (P>0.10) reproductive traits measured. Likewise, 48-H treatment did not impair health or reduce weaning weights of calves. In a separate, within-year analysis used to examine age of dam effects, productivity of 4-yr-old cows during 1984 was slightly below that of older cows for some traits. Cow age effects were not detected in other years. We conclude that control cows in our study were approaching optimum fertility and production levels in concert with their environment and that improvement beyond these levels with the treatments imposed was unlikely.
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    Influence of Ruminally Dispensed Monensin and Forage Maturity on Intake and Digestion.
    (1993-05) Fredrickson, Eddie L; Galyean, M. L.; Branine, M. E.; Sowell, Bok F.; Wallace, Joe D.
    Eight ruminally cannulated crossbred steers (average weight 336 kg) grazing native blue grama (Bouteloua gracilis [H.B.K.]) rangeland were used in a repeated measures design to evaluate effects of monensin ruminal delivery devices (MRDD) and forage phenology on ruminal digestion. Three periods were assessed: mid-August (Aug.), early October (Oct.), and mid-November (Nov.). One MRDD was placed in the reticulum of 4 steers via the ruminal cannula 21 days before each period. Intake was estimated using total fecal collections. Diet samples were collected using 3 esophageally fistualted steers. Ruminal fill was measured by ruminal exacuation; rate and extent of in situ ruminal neutral detergent fiber disappearance were estimated before ruminal evacuations. Ruminal passage rates, retention time, and apparent total tract organic matter digestibility were estimated using indigestible neutral detergent fiber. In vitro organic matter disappearance of esophageal masticate did not differ (P>.05) in Aug. and Oct. (average of 53.7%), but declined (P<.05) in Nov. (48.7%), whereas organic matter digestibility was greater (P<.10) in Aug. (62.3%) than in either Oct. (55.2%) or Nov. (53.9%). Release of monensin from the bolus (68 mg day-1) was less than expected (100 mg day-1). Intake, organic matter digestibility, ruminal passage rates, retention time, pH, and ammonia were not affected (P>.10) by MRDD. In situ neutral detergent fiber disappearance at 96 hours was decreased (P<.10) by MRDD (68 vs 65% for control and MRDD, respectively). As the grazing season progressed, intake declined (P<.10), whereas ruminal fill and retention time increased (P<.05), and passage rate of indigestible neutral detergent fiber decreased (P<.05). At 48 hours in situ neutral detergent fiber was greatest (P<.05) in Aug. and least (P<.05) in Nov.
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    Liquid supplementation for ruminants fed low-quality forage diets: a review
    (1995-09) Bowman, J. G. P.; Sowell, Bok F.; Paterson, J. A.
    Forty-three studies involving liquid supplementation of cattle and sheep consuming low-quality forages were identified, summarized in tabular form and reviewed. All studies that could be found containing animal gain, forage intake and (or) supplement consumption with molasses-urea supplements under grazing conditions were reviewed. Seven studies were found which compared forage intake or animal performance by animals fed hay or straw and molasses-urea supplements with unsupplemented animals. Molasses-urea supplements did not increase forage intake or animal performance compared with unsupplemented animals in five of the seven studies. Thirteen studies were identified which evaluated performance of grazing animals receiving molasses-urea supplements compared with unsupplemented animals. Seven of these 13 grazing studies reported improved live weight change when animals received molasses-urea supplements. Only two grazing studies were found which evaluated forage intake by animals consuming molasses-urea supplements and compared it with unsupplemented animals. Both studies found no effect. Five of six studies identified that compared molasses-urea supplements with dry supplements, forage intake or animal live weight change were not increased by molasses-urea supplements over dry supplements. Most authors concluded that feeding molasses-urea supplements to grazing ruminants was not as profitable as feeding dry supplements; however, few studies reported economic data. Studies demonstrated that level of molasses and nitrogen influenced animal performance. Asynchrony between molasses and nitrogen resulted in animal weight loss. Most positive animal responses were seen with a combination of high levels of molasses and nitrogen. However, these results may have been influenced by supplement formulation. Performance and intake results were confounded by pasture condition, forage quality, animal variation and supplement delivery system. In four studies that measured supplement intake by individual animals, between 1 and 20% of experimental animals refused to consume any molasses-urea supplement. Quantification of supplement intake and animal feeding behavior has not been adequately addressed in the literature.
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    Bio-Priming Seed Treatment for Biological Control of Pythium Ultimum Preemergence Damping-Off in SH-2 Sweet Corn
    (American Phytopathological Society, 1990) Callan, Nancy W.; Mathre, Don; Miller, James B.
    Sweet corn (Zea mays) with the shZ gene for enhanced sugar content is highly susceptible to preemergence damping-off, caused primarily by Pythium ultimum in Montana’s Bìtterroot Valley. Rhizosphere bacteria that adhered to hyphae of P. ultimum and were antagonistic to the growth of this pathogen were isolated from Bitterroot Valley soils. An isolate of Pseudomonas fluorescens, AB254, provided superior seed protection from Pythium damping-off in naturally infested soils. At least 1 X 100,000,000 cfu per seed of AB254 was needed to achieve maximum protection. In a process we have termed “bio-priming,” dry seed was coated with P. fluorescens AB254 and allowed to imbibe water under warm temperatures until a 35-40% moisture content was achieved. During bio-priming, bacterial populations increased from 10 to over l0,000­-fold, depending on initial inoculum level. Bio-priming provided protection against damping-off as good as or better than seed treatment with metalaxyl when the seeds were planted in cold soil. This process may be of interest and value to growers who wish to plant sweet corn or other temperature-sensitive crops into cold soils where damping-off is a problem and the use of chemical seed treatments is not desired.
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    Combined Biological and Chemical Seed Treatments for Control of Two Seedling Diseases of SH2 Sweet Corn
    (American Phytopathological Society, 1995) Mathre, Don; Johnston, Robert H.; Callan, Nancy W.; Mohan, S.K.; Martin, John M.
    Experiments were conducted in both the glasshouse and in the to determine if biological and chemical control agents could be used together on sweet com (Zea mays L.) seed to control Pythium ultimum damping-off or Penicillium oxalicum seedling disease. Pseudomonas aureofaciens AB254 and Pseudomonas sp. AB842 were used for control of P. ultimum and P. oxalicum, respectively. Metalaxyl seed treatment for control of P. ultimum was used at rates from 100 to 0.01% of the recommended rate either alone or in combination with P. aureofaciens AB254. Imazalil seed treatment for control of P. oxalicum was used at rates from 100 to 1% of the recommended rate either alone or in combination with Pseudomonas sp. AB842. In field tests, combining a chemical treatment with the biological agent did not affect the efficacy of disease control. Nor did combining low rates of chemical with the biocontrol agent increase the efficacy or reliability of disease control.
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    From Discovery to Use: Traversing the World of Commercializing Biocontrol Agents for Plant Disease Control
    (American Phytopathological Society, 1999) Mathre, Don; Cook, R. J.; Callan, Nancy W.
    Microorganisms play an enormously important role in plant disease control. As naturally occurring resident antagonists, they can be managed or exploited to achieve the desired results. They are responsible for the “crop rotation effect,” which is possibly still the single most important disease management tool used worldwide. On the other hand, the addition of organic materials such as compost, barnyard manure, and green manure is known to intensify the soil-sanitizing benefits of resident antagonists so much that it is sometimes possible to eliminate the need for crop rotation. Entomology is commonly used as the standard for success with biological control agents introduced into the environment. Compared case-for-case, plant pathology is a barely-visible distant second to entomology. In fact, we would have to say it is a distant third, since there are more successful cases with introduced biological control agents of weeds—herbivorous insects and pathogens—than of plant diseases. On the other hand, if we narrow the comparison to biological control with introduced micro-organisms, plant pathology begins to look quite respectable. Examples of biological control sparked the current and much more successful effort with plant-associated microorganisms as agents introduced for biological control of plant pathogens.
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