Volatile fuel & organic compound production by Ascocoryne sarcoides : exploration of environmental variables and analytical methods

dc.contributor.advisorChairperson, Graduate Committee: Brent M. Peytonen
dc.contributor.authorMallette, Natasha Dawnen
dc.contributor.otherW. Berk Knighton, Gary A. Strobel, Ross P. Carlson and Brent M. Peyton were co-authors of the article, 'Resolution of volatile fuel compound profiles from Ascocoryne sarcoides: a comparison by proton transfer reaction-mass spectrometry and solid phase microextraction gas chromatography-mass spectrometry' in the journal 'AMB express' which is contained within this thesis.en
dc.contributor.otherElle M. Pankratz, Albert E. Parker, Gary A. Strobel, Scott C. Busse, Ross P. Carlson and Brent M. Peyton were co-authors of the article, 'Evaluation of cellulose as a substrate for hydrocarbon fuel production by Ascocoryne sarcoides (NRRL 50072)' submitted to the journal 'Journal of sustainable bioenergy systems' which is contained within this thesis.en
dc.description.abstractAscocoryne sarcoides is an endophytic filamentous fungus isolated from Northern Patagonia that can produce petroleum-like fuel compounds directly from cellulose (Strobel et al., 2008). The aim of this project was to study volatile organic compound production by A. sarcoides. The project is different from many other cellulosic biofuel projects that explore alcohol production from pre-treated biomass fermentation. It focuses on the ability of A. sarcoides to convert cellulose and related simple sugars to fuel-related hydrocarbons. The influence of environmental conditions on the growth characteristics and compounds produced from normal metabolic processes was explored. Quantification of volatile production from growth on glucose showed the major compounds were ethanol and acetaldehyde, with the remainder of volatiles near 2 ppm from a continuously aerated culture. These volatiles included compounds of fuel-interest such as benzaldehyde, nonanal, 1-octen-3-ol and 1-butanol, 3-methyl-. Notable compounds with fuel related properties included isopentane, d-limonene, and cyclopropane, propyl-. These compounds all have octane ratings greater than 90 and enthalpies of combustion greater than 3200 kJ mol -1. Oxygen availability influenced the type and number of volatile compounds produced. For example, growth on a soluble cellulose substrate produced greater numbers of volatile compounds at oxygen limited concentrations and more alcohols, alkanes, aromatics, ketones, and esters were identified from mass spectrometry data. In addition, the oxygen availability influenced growth characteristics with a starting oxygen concentration of 7% was low enough to greatly inhibit growth. The growth substrate had a marked influence on the volatile compounds produced. A. sarcoides growth on microcrystalline cellulose produced a greater variety of hydrocarbon compounds compared to growth on glucose, and the highest yield of volatile organics was estimated at 105 mg (g biomass) -1 from the cellulose substrate. The fungus A. sarcoides demonstrated production of valuable fuel compounds on multiple carbon sources. Further work should carry on the analysis of culturing conditions and include evaluation of hydrocarbon yields on pre-treated cellulosic biomass. Studies of this nature should continue to advance knowledge of fungal biological potential for industrial processes.en
dc.publisherMontana State University - Bozeman, College of Engineeringen
dc.rights.holderCopyright 2013 by Natasha Dawn Malletteen
dc.subject.lcshBiomass energyen
dc.subject.lcshVolatile organic compoundsen
dc.titleVolatile fuel & organic compound production by Ascocoryne sarcoides : exploration of environmental variables and analytical methodsen
dc.title.alternativeVolatile fuel and organic compound production by Ascocoryne sarcoides: exploration of environmental variables and analytical methodsen
thesis.degree.committeemembersMembers, Graduate Committee: Ross Carlson; Phillip S. Stewart; Abigail Richards; Gary Strobelen
thesis.degree.departmentChemical & Biological Engineering.en


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