Kinetics of Calcite Precipitation by Ureolytic Bacteria under Aerobic and Anaerobic Conditions

dc.contributor.authorMitchell, Andrew C.
dc.contributor.authorEspinosa-Ortiz, Erika J.
dc.contributor.authorParks, Stacy L.
dc.contributor.authorPhillips, Adrienne J.
dc.contributor.authorCunningham, Alfred B.
dc.contributor.authorGerlach, Robin
dc.description.abstractThe kinetics of urea hydrolysis (ureolysis) and induced calcium carbonate (CaCO3) precipitation for engineering use in the subsurface was investigated under aerobic conditions using Sporosarcina pasteurii (ATCC strain 11859) as well as Bacillus sphaericus strains 21776 and 21787. All bacterial strains showed ureolytic activity inducing CaCO3 precipitation aerobically. Rate constants not normalized to biomass demonstrated slightly higher-rate coefficients for both ureolysis (kurea) and CaCO3 precipitation (kprecip) for B. sphaericus 21776 (kurea=0.10±0.03 h−1, kprecip=0.60±0.34 h−1) compared to S. pasteurii (kurea=0.07±0.02 h−1, kprecip=0.25±0.02 h−1), though these differences were not statistically significantly different. B. sphaericus 21787 showed little ureolytic activity but was still capable of inducing some CaCO3 precipitation. Cell growth appeared to be inhibited during the period of CaCO3 precipitation. Transmission electron microscopy (TEM) images suggest this is due to the encasement of cells and was reflected in lower kurea values observed in the presence of dissolved Ca. However, biomass regrowth could be observed after CaCO3 precipitation ceased, which suggests that ureolysis-induced CaCO3 precipitation is not necessarily lethal for the entire population. The kinetics of ureolysis and CaCO3 precipitation with S. pasteurii was further analyzed under anaerobic conditions. Rate coefficients obtained in anaerobic environments were comparable to those under aerobic conditions; however, no cell growth was observed under anaerobic conditions with NO−3, SO2−4 or Fe3+ as potential terminal electron acceptors. These data suggest that the initial rates of ureolysis and ureolysis-induced CaCO3 precipitation are not significantly affected by the absence of oxygen but that long-term ureolytic activity might require the addition of suitable electron acceptors. Variations in the ureolytic capabilities and associated rates of CaCO3 precipitation between strains must be fully considered in subsurface engineering strategies that utilize microbial amendments.en_US
dc.identifier.citationMitchell, Andrew C., Erika J. Espinosa-Ortiz, Stacy L. Parks, Adrienne J. Phillips, Alfred B. Cunningham, Robin Gerlach, “Kinetics of Calcite Precipitation by Ureolytic Bacteria under Aerobic and Anaerobic Conditions,” Biogeosciences, May 2019, 16:2147-2161 DOI: 10.5194/bg-16-2147-2019.en_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.titleKinetics of Calcite Precipitation by Ureolytic Bacteria under Aerobic and Anaerobic Conditionsen_US
mus.contributor.orcidMitchell, Andrew C.|0000-0001-9749-5326en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentCivil Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US


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