Determination of critical micelle concentration of an amphiphilic siderophore

dc.contributor.advisorChairperson, Graduate Committee: Abigail Richardsen
dc.contributor.authorMousseau, Kenneth Scotten
dc.date.accessioned2013-06-25T18:39:00Z
dc.date.available2013-06-25T18:39:00Z
dc.date.issued2009en
dc.description.abstractThe sodachelins are a group of six amphiphilic siderophores produced by a halophilic bacterium. Amphiphilic siderophores, such as the sodachelins, are important in the solubilization and sequestration of iron related to microbial metabolism and are also unique in their ability to form micelle and vesicular structures. This professional paper describes siderophore importance in iron bioavailability, siderophore chemistry and biological function and a thermodynamic analysis of forces that drive micellization and vesicle formation. A description of experiments conducted to isolate, separate and purify the sodachelins for the purpose of measuring their critical micelle concentration (CMC) follows the review of literature. Initial siderophore isolation was achieved using XAD-2 resin to generate a crude extract. This crude extract was then purified by HPLC, and the measurement of CMC of a single siderophore, sodachelin E, was performed with a tensiomat instrument. Crude separation by XAD resin was proven successful; XAD resin has a strong affinity for siderophores as shown by experiment with the siderophore desferrioxamine B (DFB) as a control. Purification of the crude siderophore extract by only one pass on the HPLC was proven insufficient to generate a single, pure siderophore. At least a second pass on the HPLC is required to remove all contaminants. The protocol developed for CMC analysis is consistent and accurate based on a sodium dodecyl sulfate (SDS) control experiment. An approximate CMC value of 140 micron for sodachelin E was obtained, however, HPLC analysis showed contamination of another sodachelin and possible other organic solutes, indicating that this value may be inaccurate.en
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/1907en
dc.language.isoenen
dc.publisherMontana State University - Bozeman, College of Engineeringen
dc.rights.holderCopyright 2009 by Kenneth Scott Mousseauen
dc.subject.lcshSurface tensionen
dc.subject.lcshSiderophoresen
dc.subject.lcshSurface active agentsen
dc.subject.lcshMicellesen
dc.titleDetermination of critical micelle concentration of an amphiphilic siderophoreen
dc.typeProfessional Paperen
thesis.catalog.ckey1473873en
thesis.degree.committeemembersMembers, Graduate Committee: Jeffrey Heys; Paul Gannonen
thesis.degree.departmentChemical & Biological Engineering.en
thesis.degree.genreProfessional Paperen
thesis.degree.nameMSen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage70en

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
MousseauK1209.pdf
Size:
682.72 KB
Format:
Adobe Portable Document Format
Copyright (c) 2002-2022, LYRASIS. All rights reserved.