Color measurement as a means of quantifying surface biofouling

dc.contributor.authorPitts, Betsey
dc.contributor.authorHamilton, Martin A.
dc.contributor.authorMcFeters, Gordon A.
dc.contributor.authorStewart, Philip S.
dc.contributor.authorWillse, Alan Ray
dc.contributor.authorZelver, Nick
dc.date.accessioned2018-02-12T22:18:14Z
dc.date.available2018-02-12T22:18:14Z
dc.date.issued1998-11
dc.description.abstractLaboratory reactors fitted with removable ceramic porcelain growth surfaces were inoculated with a consortium of biofilm forming environmental isolates. A Minolta colorimeter CR-200 (Minolta Camera Co., Ltd, Ramsey, NJ) was used in conjunction with a specially designed adapter to evaluate the reflective color of the porcelain disks as biofilm accumulated on them. Areal viable cell counts were monitored over a period of eleven days in two separate experiments and direct color measurements of the untreated, microbially fouled test surfaces were collected. This colorimetric assay was both non-destructive and immediate. A strong linear relationship between log cell density and log color change was observed. The Pearson product moment correlation coefficient for all 45 observations combined was r=0.95. Separate regression lines for each experiment were not significantly different (P=0.19). When adjusted for time, the (partial) correlation coefficient between log cell density and log color change was r=0.87, which suggests that the relationship between the two measures can not be explained by their mutual dependence on time. Reflective color measurement provided a rapid, non-destructive and quantitative measure of biofilm accumulation.en_US
dc.identifier.citationPitts, B., M.A. Hamilton, G.A. McFeters, P.S. Stewart, A. Willse, and N. Zelver, "Color Measurement as a Means of Quantifying Surface Biofouling," Journal of Microbiological Methods, 34:143-149 (1998).en_US
dc.identifier.issn0167-7012
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/14361
dc.titleColor measurement as a means of quantifying surface biofoulingen_US
dc.typeArticleen_US
mus.citation.extentfirstpage143en_US
mus.citation.extentlastpage149en_US
mus.citation.issue2en_US
mus.citation.journaltitleJournal of Microbiological Methodsen_US
mus.citation.volume34en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US
mus.data.thumbpage5en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1016/s0167-7012(98)00082-7en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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