Hematoloechus sp. attachment shifts endothelium in vivo from pro- to anti-inflammatory profile in Rana pipiens: evidence from systemic and capillary physiology

dc.contributor.authorWilliams, Donna A.
dc.contributor.authorFlood, Mary H.
dc.date.accessioned2023-11-21T19:00:12Z
dc.date.available2023-11-21T19:00:12Z
dc.date.issued2023-08
dc.description© American Physiological Societyen_US
dc.description.abstractThis prospective, descriptive study focused on lung flukes (Hematoloechus sp., H) and their impact on systemic and individual capillary variables measured in pithed Rana pipiens, a long-standing model for studies of capillary physiology. Three groups were identified based on Hematoloechus attachment: no Hematoloechus (No H), Hematoloechus not attached (H Not Att), and Hematoloechus attached (H Att). Among 38 descriptive, cardiovascular, and immunological variables, 18 changed significantly with H. Symptoms of H included weight loss, elevated immune cells, heart rate variability, faster coagulation, lower hematocrit, and fluid accumulation. Important capillary function discoveries included median baselines for hydraulic conductivity (Lp) of 7.0 (No H), 12.4 (H Not Att), and 4.2 (H Att) × 10−7 cm·s−1·cmH2O−1 (P < 0.0001) plus seasonal adaptation of sigma delta pi [σ(πc–πi), P = 0.03]. Pro- and anti-inflammatory phases were revealed for Lp and plasma nitrite/nitrate concentration ([NOx]) in both H Not Att and H Att, whereas capillary wall tensile strength increased in the H Att. H attachment was advantageous for the host due to lower edema and for the parasite via a sustained food source illustrating an excellent example of natural symbiosis. However, H attachment also resulted in host weight loss: in time, a conundrum for the highly dependent parasite. The study increases overall knowledge of Rana pipiens by revealing intriguing effects of H and previously unknown, naturally occurring seasonal changes in many variables. The data improve Rana pipiens as a general scientific and capillary physiology model. Diseases of inflammation and stroke are among the clinical applications.en_US
dc.identifier.citationWilliams, D. A., & Flood, M. H. (2023). Hematoloechus sp. attachment shifts endothelium in vivo from pro-to anti-inflammatory profile in Rana pipiens: evidence from systemic and capillary physiology. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 325(2), R133-R153.en_US
dc.identifier.issn0363-6119
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/18233
dc.language.isoen_USen_US
dc.publisherAmerican Physiological Societyen_US
dc.rightscopyright American Physiological Society 2023en_US
dc.rights.urihttps://web.archive.org/web/20200225084001/https://journals.physiology.org/author-info.permissionsen_US
dc.subjecthydrationen_US
dc.subjectlower vertebratesen_US
dc.subjectnutritionen_US
dc.subjectpulmonaryen_US
dc.subjectparasitologyen_US
dc.titleHematoloechus sp. attachment shifts endothelium in vivo from pro- to anti-inflammatory profile in Rana pipiens: evidence from systemic and capillary physiologyen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage21en_US
mus.citation.issue2en_US
mus.citation.journaltitleAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiologyen_US
mus.citation.volume325en_US
mus.data.thumbpage10en_US
mus.identifier.doi10.1152/ajpregu.00041.2023en_US
mus.relation.collegeCollege of Nursingen_US
mus.relation.departmentNursing.en_US
mus.relation.universityMontana State University - Bozemanen_US

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