Honey bee infecting Lake Sinai viruses

dc.contributor.authorDaughenbaugh, Katie F.
dc.contributor.authorMartin, Madison
dc.contributor.authorBrutscher, Laura M.
dc.contributor.authorCavigli, Ian
dc.contributor.authorGarcia, Emma
dc.contributor.authorLavin, Matthew
dc.contributor.authorFlenniken, Michelle L.
dc.date.accessioned2019-02-13T19:17:26Z
dc.date.available2019-02-13T19:17:26Z
dc.date.issued2015-06
dc.description.abstractHoney bees are critical pollinators of important agricultural crops. Recently, high annual losses of honey bee colonies have prompted further investigation of honey bee infecting viruses. To better characterize the recently discovered and very prevalent Lake Sinai virus (LSV) group, we sequenced currently circulating LSVs, performed phylogenetic analysis, and obtained images of LSV2. Sequence analysis resulted in extension of the LSV1 and LSV2 genomes, the first detection of LSV4 in the US, and the discovery of LSV6 and LSV7. We detected LSV1 and LSV2 in the Varroa destructor mite, and determined that a large proportion of LSV2 is found in the honey bee gut, suggesting that vector-mediated, food-associated, and/or fecal-oral routes may be important for LSV dissemination. Pathogen-specific quantitative PCR data, obtained from samples collected during a small-scale monitoring project, revealed that LSV2, LSV1, Black queen cell virus (BQCV), and Nosema ceranae were more abundant in weak colonies than strong colonies within this sample cohort. Together, these results enhance our current understanding of LSVs and illustrate the importance of future studies aimed at investigating the role of LSVs and other pathogens on honey bee health at both the individual and colony levels.en_US
dc.description.sponsorshipMontana Department of Agriculture Specialty Crop Block Grant Program (1450687); National Institutes of Health IDeA Program COBRE grant GM110732; National Science Foundation EPSCoR NSF-IIA-1443108; Montana State University Agricultural Experiment Station; Project Apis m.; Howard Hughes Medical Institute Undergraduate Scholars Program (52006931); United States Department of Agriculture; National Institute of Food and Agriculture; Agriculture and Food Research Initiative (USDA-NIFA-AFRI); National Honey Board; Montana State Beekeepers Associationen_US
dc.identifier.citationDaughenbaugh, Katie, Madison Martin, Laura Brutscher, Ian Cavigli, Emma Garcia, Matt Lavin, and Michelle Flenniken. “Honey Bee Infecting Lake Sinai Viruses.” Viruses 7, no. 6 (June 23, 2015): 3285–3309. doi:10.3390/v7062772.en_US
dc.identifier.issn1999-4915
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/15256
dc.language.isoenen_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleHoney bee infecting Lake Sinai virusesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage3285en_US
mus.citation.extentlastpage3309en_US
mus.citation.issue6en_US
mus.citation.journaltitleVirusesen_US
mus.citation.volume7en_US
mus.contributor.orcidLavin, Matthew|0000-0003-4205-1802en_US
mus.data.thumbpage5en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.3390/v7062772en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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