Formaldehyde as a carbon and electron shuttle between autotroph and heterotroph populations in acidic hydrothermal vents of Norris Geyser Basin, Yellowstone National Park

dc.contributor.authorMoran, James J.
dc.contributor.authorWhitmore, Laura M.
dc.contributor.authorIsern, Nancy G.
dc.contributor.authorRomine, Margaret F.
dc.contributor.authorRiha, Krystin M.
dc.contributor.authorInskeep, William P.
dc.contributor.authorKreuzer, Helen W.
dc.date.accessioned2016-12-12T18:41:11Z
dc.date.available2016-12-12T18:41:11Z
dc.date.issued2016-05
dc.description.abstractThe Norris Geyser Basin in Yellowstone National Park contains a large number of hydrothermal systems, which host microbial populations supported by primary productivity associated with a suite of chemolithotrophic metabolisms. We demonstrate that Metallosphaera yellowstonensis MK1, a facultative autotrophic archaeon isolated from a hyperthermal acidic hydrous ferric oxide (HFO) spring in Norris Geyser Basin, excretes formaldehyde during autotrophic growth. To determine the fate of formaldehyde in this low organic carbon environment, we incubated native microbial mat (containing M. yellowstonensis) from a HFO spring with 13C-formaldehyde. Isotopic analysis of incubation-derived CO2 and biomass showed that formaldehyde was both oxidized and assimilated by members of the community. Autotrophy, formaldehyde oxidation, and formaldehyde assimilation displayed different sensitivities to chemical inhibitors, suggesting that distinct sub-populations in the mat selectively perform these functions. Our results demonstrate that electrons originally resulting from iron oxidation can energetically fuel autotrophic carbon fixation and associated formaldehyde excretion, and that formaldehyde is both oxidized and assimilated by different organisms within the native microbial community. Thus, formaldehyde can effectively act as a carbon and electron shuttle connecting the autotrophic, iron oxidizing members with associated heterotrophic members in the HFO community.en_US
dc.description.sponsorshipJoint Genome Institute (DE-AC02-05CH11231); Community Sequencing Project to WPI (CSP 787081); permit (permit no. YELL-2014-SCI-5068)en_US
dc.identifier.citationMoran, James J. , Laura M. Whitmore, Nancy G. Isern, Margaret F. Romine, Krystin M. Riha, William P. Inskeep, and Helen W. Kreuzer. "Formaldehyde as a carbon and electron shuttle between autotroph and heterotroph populations in acidic hydrothermal vents of Norris Geyser Basin, Yellowstone National Park." Extremophiles 20, no. 3 (May 2016): 291-299. DOI:https://dx.doi.org/10.1007/s00792-016-0821-2.en_US
dc.identifier.issn1431-0651
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/12393
dc.language.isoen_USen_US
dc.subjectAcidophilicen_US
dc.subjectCarbon transferen_US
dc.subjectYellowstone National Parken_US
dc.subjectArchaeaen_US
dc.subjectCarbon cycleen_US
dc.titleFormaldehyde as a carbon and electron shuttle between autotroph and heterotroph populations in acidic hydrothermal vents of Norris Geyser Basin, Yellowstone National Parken_US
dc.typeArticleen_US
mus.citation.extentfirstpage291en_US
mus.citation.extentlastpage299en_US
mus.citation.issue3en_US
mus.citation.journaltitleExtremophilesen_US
mus.citation.volume20en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doihttps://dx.doi.org/10.1007/s00792-016-0821-2en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US

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