Expression and purification of two CRISPR-CAS proteins, Csm3 and Csm5 from Mycobacterium tuberculosis

dc.contributor.advisorChairperson, Graduate Committee: C. Martin Lawrenceen
dc.contributor.authorHashimi, Marziahen
dc.date.accessioned2017-05-11T14:45:42Z
dc.date.available2017-05-11T14:45:42Z
dc.date.issued2015en
dc.description.abstractOne third of the World's population is infected with tuberculosis (TB). TB disease is caused by bacterium called Mycobacterim tuberculosis, which is a facultative intracellular parasite that is transferred through the air from one person to another in close contact. A six month course of four antimicrobial drugs is the only current treatment for drug-sensitive TB. However, multi-drug resistance TB is difficult to treat. Phage therapy might be one answer as a treatment for multi-drug resistance TB. In order for phage therapy to have a chance against TB, the immune system of bacteria, known as CRISPR/Cas needs to be inhibited. Our lab has taken a structural and biochemical approach to try to understand the CRISPR/Cas system in M. tuberculosis. We have cloned, expressed, and purified individual Csm proteins from the H37Rv M. tuberculosis strain. Two Csm protein, Csm3 and Csm5 were successfully purified to homogeneity in yields suitable for structure and biochemical studies. While to date, each has failed to produce crystals, the ability to the express and purify each of these proteins will allow further biochemical characterization of Csm3 and Csm5.en
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/12734en
dc.language.isoenen
dc.publisherMontana State University - Bozeman, College of Letters & Scienceen
dc.rights.holderCopyright 2015 by Marziah Hashimien
dc.subject.lcshTuberculosisen
dc.subject.lcshDrug resistanceen
dc.subject.lcshBacteriophagesen
dc.subject.lcshCRISPR (Genetics)en
dc.titleExpression and purification of two CRISPR-CAS proteins, Csm3 and Csm5 from Mycobacterium tuberculosisen
dc.typeThesisen
mus.data.thumbpage39en
thesis.degree.committeemembersMembers, Graduate Committee: Mark J. Young; Brian Bothner.en
thesis.degree.departmentChemistry & Biochemistry.en
thesis.degree.genreThesisen
thesis.degree.nameMSen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage62en

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