Metagenomes from high-temperature chemotrophic systems reveal geochemical controls on microbial community structure and function

dc.contributor.authorInskeep, William P.
dc.contributor.authorRusch, Douglas B.
dc.contributor.authorJay, Zackary J.
dc.contributor.authorHerrgard, Markus J.
dc.contributor.authorKozubal, Mark A.
dc.contributor.authorRichardson, Toby H.
dc.contributor.authorMacur, Richard E.
dc.contributor.authorHamamura, Natsuko
dc.contributor.authorJennings, Ryan deM.
dc.contributor.authorFouke, Bruce W.
dc.contributor.authorReysenbach, Anna-Louise
dc.contributor.authorRoberto, Frank
dc.contributor.authorYoung, Mark J.
dc.contributor.authorSchwartz, Ariel
dc.contributor.authorBoyd, Eric S.
dc.contributor.authorBadger, Jonathan H.
dc.contributor.authorMathur, Eric J.
dc.contributor.authorOrtmann, Alice C.
dc.contributor.authorBateson, Mary M.
dc.contributor.authorGeesey, Gill G.
dc.contributor.authorFrazier, Marvin
dc.description.abstractThe Yellowstone caldera contains the most numerous and diverse geothermal systems on Earth, yielding an extensive array of unique high-temperature environments that host a variety of deeply-rooted and understudied Archaea, Bacteria and Eukarya. The combination of extreme temperature and chemical conditions encountered in geothermal environments often results in considerably less microbial diversity than other terrestrial habitats and offers a tremendous opportunity for studying the structure and function of indigenous microbial communities and for establishing linkages between putative metabolisms and element cycling. Metagenome sequence (14–15,000 Sanger reads per site) was obtained for five high-temperature (>65°C) chemotrophic microbial communities sampled from geothermal springs (or pools) in Yellowstone National Park (YNP) that exhibit a wide range in geochemistry including pH, dissolved sulfide, dissolved oxygen and ferrous iron. Metagenome data revealed significant differences in the predominant phyla associated with each of these geochemical environments. Novel members of the Sulfolobales are dominant in low pH environments, while other Crenarchaeota including distantly-related Thermoproteales and Desulfurococcales populations dominate in suboxic sulfidic sediments. Several novel archaeal groups are well represented in an acidic (pH 3) Fe-oxyhydroxide mat, where a higher O2 influx is accompanied with an increase in archaeal diversity. The presence or absence of genes and pathways important in S oxidation-reduction, H2-oxidation, and aerobic respiration (terminal oxidation) provide insight regarding the metabolic strategies of indigenous organisms present in geothermal systems. Multiple-pathway and protein-specific functional analysis of metagenome sequence data corroborated results from phylogenetic analyses and clearly demonstrate major differences in metabolic potential across sites. The distribution of functional genes involved in electron transport is consistent with the hypothesis that geochemical parameters (e.g., pH, sulfide, Fe, O2) control microbial community structure and function in YNP geothermal springs.en_US
dc.description.sponsorshipU.S. National Aeronautics and Space Administration Exobiology Program NAG5-8807, NNG04GR46G), U.S. National Science Foundation Research Coordination Network BIO 0342269; Montana Agricultural Experiment Station Project 911398en_US
dc.identifier.citationInskeep, William P., Rusch, Douglas B., Jay, Zackary J., Herrgard, Markus J., Kozubal, Mark A., Richardson, Toby H., Macur, Richard E., Hamamura, Natsuko, Jennings, Ryan deM., Fouke, Bruce W., Reysenbach, Anna-Louise, Roberto, Frank, Young, Mark J., Schwartz, Ariel, Boyd, Eric S., Badger, Jonathan H., Mathur, Eric J., Ortmann, Alice C., Bateson, Mary M., Geesey, Gill G., Frazier, Marvin. Metagenomes from high-temperature chemotrophic systems reveal geochemical controls on microbial community structure and function. PLoS One. 2010 Mar 19;5(3):e9773. doi: 10.1371/journal.pone.0009773.en_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.titleMetagenomes from high-temperature chemotrophic systems reveal geochemical controls on microbial community structure and functionen_US
mus.citation.journaltitlePLoS Oneen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.researchgroupMT INBRE Bioinformatics and Biostatistics Core.en_US
mus.relation.researchgroupThermal Biology Institute (TBI).en_US
mus.relation.universityMontana State University - Bozemanen_US


Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
6.53 MB
Adobe Portable Document Format
Item Status Item Bitstreams Item Metadata View Item Curate Metagenomes from high-temperature chemotrophic systems reveal geochemical controls on microbial community structure and function (PDF)

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
826 B
Item-specific license agreed upon to submission
Copyright (c) 2002-2022, LYRASIS. All rights reserved.