Browsing by Author "Buyukyoruk, Murat"
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Item Type I-F CRISPR-Cas provides protection from DNA, but not RNA phages(2019-10) Buyukyoruk, Murat; Wiedenheft, Blake A.The type I-F CRISPR–Cas system from Pseudomonas aeruginosa (PA14) provides sequence-specific elimination of invading DNA1,2. Recently, a paper published in Cell Research reported that this system targets mRNA through a non-canonical mechanism3. Here, we implement the proposed design rules for generating CRISPR-RNA (crRNA)-guides that target RNA and test whether the proposed RNA-targeting activity provides immunity against RNA phage infection. Our experiments reveal that the type I-F CRISPR system provides protection from DNA, but not RNA phages.