Browsing by Author "Hewitt, Tim"
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Item Stem rust resistance in wheat is suppressedby a subunit of the mediator complex(2020-02) Hiebert, Colin W.; Moscou, Matthew J.; Hewitt, Tim; Steuernagel, Burkhard; Hernandez-Pinzon, Inma; Green, Phon; Pujol, Vincent; Zhang, Peng; Rouse, Matthew N.; Jin, Yue; McIntosh, Robert A.; Upadhyaya, Narayana; Zhang, Jianping; Bhavani, Sridhar; Vrana, Jan; Karafiatova, Miroslava; Huang, Li; Fetch, Tom; Dolezel, Jaroslav; Wulff, Brande B. H.; Lagudah, Evans; Spielmeyer, WolfgangStem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.