Browsing by Author "Moffet, Matthew Durwin"

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Identifying regions of conserved synteny between pea (pisum spp.), lentil (lens spp.), and bean (phaseolus spp.)
(Montana State University - Bozeman, College of Agriculture, 2006) Moffet, Matthew Durwin; Chairperson, Graduate Committee: Norman Weeden.
The identification of conserved synteny in legumes can facilitate many different types of gene discovery. Techniques like marker assisted selection and the candidate gene approach can benefit greatly by identifying conserved synteny and genes located within those regions. Both Pisum and Phaseolus have detailed linkage maps, but a limited number of markers have been located in both species. In the present study I mapped 21 genes in Phaseolus vulgaris, 16 of which had already been located on the Lens and Pisum sativum linkage maps, the markers were used to look for conserved synteny between Pisum, Lens and Phaseolus. In particular, I was able to target marker/gene-rich regions of pea linkage groups V and VII, as well as pea linkage group III, with Pisum STS markers and universally designed gene-specific markers already located on the pea linkage map.