Scholarly Work - Plant Sciences & Plant Pathology
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/8870
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Item Genetic dissection of endosperm hydration in malting barley (Hordeum vulgare)(Wiley, 2023-08) Jensen, Joseph; Turner, Hannah; Lachowiec, Jennifer; Lutgen, Greg; Yin, Xiang S.; Sherman, JamieHydration of the endosperm is a critical part of the malting process that ensures proper modification of the grain. However, little is known about the genetic controls of endosperm hydration and its relationship to agronomic and malt quality traits. The extent of endosperm hydration is estimated through hydration index (HYI). We measured HYI, agronomic, and malt quality traits on a 169-line subset of the NSGC Barley Core Panel, which includes global malt lines, some dating from the inception of European breeding programmes. Utilizing GWAS, 61 QTLs were identified for HYI, dormancy, agronomic, and malt quality traits. Of these, six were found to be related to HYI and were located on 1H, 2H, 3H, 6H, and 7H. We found HYI QTLs cosegregating with kernel size and hardness (1H and 3H), malting quality (2H and 6H), and dormancy (2H and 6H). These results indicate that endosperm hydration after steeping can be improved by selecting high HYI alleles on 2H, 6H, and 7H, positively impacting malting quality without negatively impacting kernel size or dormancy.Item Historic trends and sources of year‐over‐year stability in Montana winter wheat yields(Wiley, 2023-04) Lachowiec, Jennifer; Berg, James E.; Liang, Meng; Correr, Fernando H.Producers desire cultivars that consistently perform with high yields and end-use qualities. Unlike easily recognized average yield improvements, yield stability over time is less examined, especially when considering the role of breeding relative to other factors like management and changing climatic conditions. Our study system was a 70-year historical dataset from which we estimated the year-over-year stability of winter wheat (Triticum aestivum L.) cultivars released by Montana's Agricultural Experimental Station. We examined yield stability within six locations representing diverse growing conditions across Montana and found no evidence that breeding has improved stability, that stability may be decreasing over time at one location, and that the year-over-year stability of a cultivar is sensitive to location. We examined the role of climatic conditions, including temperature, and rainfall to understand if increased climatic variability was masking improved patterns of stability. However, the lack of impact of breeding remained. These findings suggest that Montana's winter wheat may benefit from selective breeding for increased stability within locations.Item Genetic dissection of natural variation in oilseed traits of camelina by whole-genome resequencing and QTL mapping(Wiley, 2021-06) Li, Huang; Hu, Xiao; Lovell, John T.; Grabowski, Paul P.; Mamidi, Sujan; Chen, Cindy; Amirebrahimi, Mojgan; Kahanda, Indika; Mumey, Brendan; Barry, Kerrie; Kudrna, David; Schmutz, Jeremy; Lachowiec, Jennifer; Lu, ChaofuCamelina [Camelina sativa (L.) Crantz] is an oilseed crop in the Brassicaceae family that is currently being developed as a source of bioenergy and healthy fatty acids. To facilitate modern breeding efforts through marker-assisted selection and biotechnology, we evaluated genetic variation among a worldwide collection of 222 camelina accessions. We performed whole-genome resequencing to obtain single nucleotide polymorphism (SNP) markers and to analyze genomic diversity. We also conducted phenotypic field evaluations in two consecutive seasons for variations in key agronomic traits related to oilseed production such as seed size, oil content (OC), fatty acid composition, and flowering time. We determined the population structure of the camelina accessions using 161,301 SNPs. Further, we identified quantitative trait loci (QTL) and candidate genes controlling the above field-evaluated traits by genome-wide association studies (GWAS) complemented with linkage mapping using a recombinant inbred line (RIL) population. Characterization of the natural variation at the genome and phenotypic levels provides valuable resources to camelina genetic studies and crop improvement. The QTL and candidate genes should assist in breeding of advanced camelina varieties that can be integrated into the cropping systems for the production of high yield of oils of desired fatty acid composition.Item Roles of Brassinosteroids in Mitigating Heat Stress Damage in Cereal Crops(MDPI AG, 2021-03) Kothari, Aishwarya; Lachowiec, JenniferHeat stress causes huge losses in the yield of cereal crops. Temperature influences the rate of plant metabolic and developmental processes that ultimately determine the production of grains, with high temperatures causing a reduction in grain yield and quality. To ensure continued food security, the tolerance of high temperature is rapidly becoming necessary. Brassinosteroids (BR) are a class of plant hormones that impact tolerance to various biotic and abiotic stresses and regulate cereal growth and fertility. Fine-tuning the action of BR has the potential to increase cereals’ tolerance and acclimation to heat stress and maintain yields. Mechanistically, exogenous applications of BR protect yields through amplifying responses to heat stress and rescuing the expression of growth promoters. Varied BR compounds and differential signaling mechanisms across cereals point to a diversity of mechanisms that can be leveraged to mitigate heat stress. Further, hormone transport and BR interaction with other molecules in plants may be critical to utilizing BR as protective agrochemicals against heat stress. Understanding the interplay between heat stress responses, growth processes and hormone signaling may lead us to a comprehensive dogma of how to tune BR application for optimizing cereal growth under challenging environments in the field.