Theses and Dissertations at Montana State University (MSU)
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/733
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Item Characterization and assessing fungicide sensitivity of Fusarium spp. causing root rot and wilt in lentils in the northern Great Plains(Montana State University - Bozeman, College of Agriculture, 2022) Bugingo, Collins; Chairperson, Graduate Committee: Mary Burrows; This is a manuscript style paper that includes co-authored chapters.Fusarium root rot and wilt are yield limiting diseases caused by Fusarium spp. in lentils globally. Seedborne Fusarium species and races of Fusarium oxysporum f. sp. lentis (Fol) have not been characterized. The sensitivity of commonly used fungicides on prevalent species is not fully known. In 2019 and 2020 commercial lentil fields were surveyed and seed lots collected from infected patches and further isolation, morphological and molecular characterization was conducted in the laboratory. Additional isolates and lentil seed were received from Washington, Montana, Idaho, North Dakota, southern Canada, and Spain for fungicide, seedborne Fusarium spp. and race-characterization of Fol respectively. In addition to assessing presence of seedborne Fusarium pathogens on the seed coat, the cotyledon and embryo were assessed for the presence of Fusarium spp. Fungicide sensitivity was assessed using mycelial assays and an additional spore assay for pyraclostrobin. A total of 84 seed lots from the Northern Great Plains (NGP) were assayed where a total of 486 and 228 Fusarium isolates were isolated in 2019 and 2020, respectively and all the representative isolates were virulent with a 1.5-4.5 root rot severity rating. Most isolates were found in the seed coat (57- 75%), cotyledon (19-23%) and embryo (6-20%) for the 2019 and 2020 isolates, respectively. F. oxysporum and F. acuminatum were all either sensitive or intermediately sensitive to prothioconazole and ipconazole. A total of 51 isolates were race-typed and 9 races identified whereby race 7 and 1 were the predominant at 53% and 16%, respectively. This study underscores the importance of understanding Fusarium spp. composition in lentil production, role of seedborne inoculum, fungicide efficacy in root rot/wilt management and race composition upon developing effective Integrated Pest Management (IPM) strategies.Item Cytological and molecular investigations in Lens and Pisum(Montana State University - Bozeman, College of Agriculture, 2003) Walling, Jason GordonItem Identifying regions of conserved synteny between pea (pisum spp.), lentil (lens spp.), and bean (phaseolus spp.)(Montana State University - Bozeman, College of Agriculture, 2006) Moffet, Matthew Durwin; Chairperson, Graduate Committee: Norman Weeden.The identification of conserved synteny in legumes can facilitate many different types of gene discovery. Techniques like marker assisted selection and the candidate gene approach can benefit greatly by identifying conserved synteny and genes located within those regions. Both Pisum and Phaseolus have detailed linkage maps, but a limited number of markers have been located in both species. In the present study I mapped 21 genes in Phaseolus vulgaris, 16 of which had already been located on the Lens and Pisum sativum linkage maps, the markers were used to look for conserved synteny between Pisum, Lens and Phaseolus. In particular, I was able to target marker/gene-rich regions of pea linkage groups V and VII, as well as pea linkage group III, with Pisum STS markers and universally designed gene-specific markers already located on the pea linkage map.