Theses and Dissertations at Montana State University (MSU)
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Item Microbiological findings of pollution studies on the East Gallatin River and its tributaries(Montana State University - Bozeman, College of Agriculture, 1968) Ehlke, Theodore AllenItem The Limulus lysate assay as a rapid and sensitive test of bacterial water quality(Montana State University - Bozeman, College of Agriculture, 1975) Evans, Thomas MorganItem A classification according to the mammalian sources of coliform organisms isolated from a remote mountain stream(Montana State University - Bozeman, College of Agriculture, 1972) Wilson, Gareth AllenItem Contributions of chemoautotrophic bacteria to the acid thermal waters of the geyser springs group in Yellowstone National Park(Montana State University - Bozeman, College of Agriculture, 1966) Brierley, James AlanItem Recovery characteristics of bacteria injured in the natural aquatic environment(Montana State University - Bozeman, College of Agriculture, 1974) Bissonnette, Gary K.Item Virus dynamics, archaeal populations, and water chemistry of three acidic hot springs in Yellowstone National Park(Montana State University - Bozeman, College of Letters & Science, 2005) Snyder, Jamie Christine; Chairperson, Graduate Committee: Mark J. YoungFew viruses from Archaea have been described and only a limited number have been characterized in molecular detail. Most hyperthermophilic viruses isolated from the archaeal sub-domain Crenarchaeota belong to the Sulfolobales family. We have focused on the isolation and analysis of viruses replicating in Sulfolobus. Sulfolobus is an acidophilic hyperthermophile that lives optimally at 80°C and pH 3. The SSVs (Sulfolobus spindle-shaped viruses) are the viruses most commonly isolated from thermal acidic areas around the world. SSVs are dsDNA spindle-shaped viruses with genomes of approximately 15kb. They are also the predominant virus isolated from samples collected in Yellowstone National Park (YNP). The SIRVs (Sulfolobus islandicus rodshaped viruses) are also commonly isolated from YNP. These viruses have linear dsDNA genomes of approximately 33kb. I have used these two viruses to investigate the interaction of time and viral population dynamics. For a comparison between geographically isolated acidic hot springs, we chose to study three sites in YNP.Item Biology of acid-sulfate-chloride springs in Yellowstone National Park, Wyoming, United States of America(Montana State University - Bozeman, College of Letters & Science, 2007) Boyd, Eric Stephen; Chairperson, Graduate Committee: Gill G. Geesey; Timothy McDermott (co-chair)This dissertation investigated the role of biology in several biogeochemical cycles in acid sulfate chloride (ASC) geothermal springs in Yellowstone National Park (YNP). Elemental sulfur (S°) is associated with many geothermal springs, yet little is known about the organisms involved in its cycling. The aqueous and solid phase geochemistry near the source of Dragon Spring, an ASC spring in the Norris Geyser Basin (NGB) of YNP, was used to guide the enrichment and isolation of two novel S°-reducing Crenarchaeota affiliated with the order Desulfurococcales. Both isolates are chemoorganotrophs, dependent on complex peptide-containing carbon sources, S°, and anaerobic conditions for respiration-dependent growth. Physiological characterization suggests the isolates are adapted to the physicochemical conditions of Dragon Spring which is supported by quantitative PCR analysis which indicates that the isolates represent a significant fraction of the microbial community associated with S° precipitates in several ASC geothermal springs in the NGB in YNP.