Theses and Dissertations at Montana State University (MSU)
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Item The effect of indomethacin on healthy frog capillaries following a shear stress stimulus : potential clinical implications(Montana State University - Bozeman, College of Nursing, 2010) Staigmiller, Carmen Kay; Chairperson, Graduate Committee: Elizabeth S. KinionWhen cultured endothelial cells are stimulated by shear stress they release prostaglandins. One of the effects of prostaglandin release is vasodilation. The effect of prostaglandins on hydraulic conductivity (Lp) is not known. Indomethacin is a nonsteroidal anti-inflammatory drug (NSAID) that is known to block prostaglandins. The purpose of this study was to evaluate the effect of indomethacin on endothelial cells of a true capillary. It was hypothesized that in an intact healthy capillary, superfused with indomethacin, there will be a decrease in capillary Lp after a shear stress stimulus. The mesentery of healthy North American leopard frogs (n=16) was exposed and a capillary was cannulated. Using the modified Landis technique Lp was assessed, after a shear stress stimulus, in a control capillary and a capillary that was exposed to indomethacin. There was not a significant decrease (P=0.13) in Lp when comparing the control vessels with treatment vessels. Data from this study suggest that prostaglandins are not involved with the response of Lp to shear stress in healthy frogs. Since Lp did not decrease in the presence of indomethacin, it is probable that other processes are affecting the response of the endothelium. The clinical implications of this study are important because of the potentially devastating impact of the toxic effects of NSAIDs.Item Clinical implications of indomethacin superfused over the capillaries of frogs with activated white blood cells(Montana State University - Bozeman, College of Nursing, 2010) Priebe, Milissa Ann; Chairperson, Graduate Committee: Elizabeth S. KinionTreatment for inflammation is controversial. The purpose of this study was to investigate the effect of indomethacin on capillary permeability in animals with signs of systemic inflammation. We hypothesized that the permeability of an individual capillary would be lower after a shear stress challenge in the presence of indomethacin when evidence of systemic infection was present in the animal. Frogs (n=13) were pithed and the mesentery was exposed, hydraulic conductivity (L p) was assessed at 30 cm H 2O using the modified Landis technique after an abrupt change in shear stress. Two capillaries from each frog were used; one was a control and one with indomethacin superfused over the tissue. The frogs showed a systemic infection (nitro blue tetrazolium activation) but individual capillaries had no evidence of rolling or sticking white cells. There was a significant decrease in L p (P=0.002) when comparing the control and treatment vessels. The results of the analysis indicate capillary L p assessed in mesentery of infected frogs, decreased when exposed to shear stress and indomethacin. The data imply that gaps between endothelial cells may get smaller when indomethacin is introduced into the system decreasing the flow of fluids out of the capillary.