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    Influences of increasing levels of sulfate in drinking water on the intake and use of low-quality forages by beef cattle
    (American Registry of Professional Animal Scientists, 2023-02) Wyffels, Samuel A.; Van Emon, Megan L.; Nack, Makae F.; Manoukian, Marley K.; Carlisle, Tanner J.; Davis, Noah G.; Kluth, Janessa A.; DelCurto-Wyffels, Hannah M.; DelCurto, Timothy
    Objective. This study evaluated the effects of varying sulfate concentrations of water on forage and water intake, digestibility, digestive kinetics, and rumen fermentation characteristics of cattle consuming low-quality forages provided a protein supplement, with and without salt. Materials and Methods. Eight ruminally cannulated cows (2 yr of age) were used in 2 concurrent 4 × 4 Latin squares (4 cows per square) to test the effects of increasing water sulfate concentrations on forage and water intake, digestibility, digestive kinetics, and rumen fermentation characteristics of cattle consuming low-quality forages provided protein supplement with and without salt. Within each square, cows were randomly assigned to the following treatments: (1) control (<10 mg/L sulfate); (2) 473 mg/L; (3) 946 mg/L; and (4) 1,420 mg/L. All cattle were provided a crude protein supplement at 0.18% of BW daily (0800 h daily); however, protein supplement NaCl composition differed by square (no NaCl vs. addition of 25% NaCl). Each period consisted of a 14-d adaptation period, followed by a 7-d intake and digestion period with ruminal profiles conducted on d 22 and complete ruminal evacuations on d 23, 5 h after feeding. Results and Discussion. There were no observed effects of sulfate (SO4) levels on forage intake, water intake, ruminal DM and liquid fill, ruminal DM and NDF digestibility, ruminal liquid passage rate, ruminal liquid turnover, ruminal liquid flow rate, ruminal pH, ruminal ammonia, ruminal total VFA concentrations, ruminal individual VFA concentrations, or the ruminal acetate-to-propionate ratio (P ≥ 0.16). Furthermore, the addition of 25% salt to supplement had no effect on forage intake, ruminal DM and liquid fill, DM and NDF digestibility, liquid passage rate, liquid turnover, liquid flow rate, ruminal pH, or the acetate-to-propionate ratio (P ≥ 0.24). Conversely, water intake was greater for animals provided 25% salt in supplement compared with animals not provided salt (P = 0.05). Implications and Applications. Sulfate water concentrations as high as 1,420 mg/L had minimal effects on intake, digestibility, and rumen fermentation characteristics of cattle consuming low-quality forage-based diets when provided a protein supplement containing up to 25% salt.
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    Evaluation of sustained release mineral boluses as a long-term nutrient delivery method for beef cattle
    (2021-09) Carlisle, Tanner J.; Wyffels, Samuel A.; Stafford, Steve D.; Taylor, Anna R.; Van Emon, Megan L.; DelCurto, Timothy
    Two studies were conducted to evaluate the efficacy of sustained release mineral boluses as an alternative nutrient delivery method for beef cattle. For both studies 16 ruminally-cannulated cows were used in a completely randomized design. In study 1, we evaluated degradation rates of two bolus prototypes and cow age (2-yr-old versus 3-yr-old cows) over an 87-d study period. In study 2, we evaluated two bolus types (90-d degradation target versus 180-d degradation target), as well as two diet qualities contrasting a low-quality high-fiber forage (> 600 g/kg neutral detergent fiber and < 80 g/kg crude protein, dry matter basis) and high-quality low-fiber forage (< 500 g/kg neutral detergent fiber and> 150 g/kg crude protein, dry matter basis). For both Study 1 & 2, intake and digestion periods were conducted to evaluate cow age (study 1) or diet quality (study 2) effects on intake and rumen/reticulum function. In study 1, models containing an asymptotic effect of day and an interaction between day and bolus type were the best supported of the candidate models for bolus degradation rate. Cow age did not affect (P= 0.48) bolus degradation rates ( = -0.81 ± 1.13) and degradation rates were greater (P < 0.01) for bolus prototype B compared to bolus A ( prototype B = -20.39 ± 1.13; prototype A = -9.64 ± 0.81). Bolus degradation rate displayed an asymptotic relationship (P < 0.01) to bolus surface area for prototype A ( = 5.83 ± 0.57) and a linear relationship (P < 0.01) for prototype B ( = 0.001 ± 0.0001). In study 2, models containing a linear effect of day and an interaction between day and diet were the best supported of the candidate models for the degradation rate of the 90-d and 180-d prototype. In addition, both bolus protoypes displayed a diet quality × time interaction (P < 0.01) for bolus degradation rate. Cattle treated with the 90-d bolus and fed a high-quality diet had a greater (P < 0.01) degradation rate ( High-quality = -2.64 ± 0.08; Low-quality = -1.97 ± 0.10) than the cows that were fed a low-quality diet. In contrast, cattle treated with the 180-d bolus had an inverse effect (P < 0.01), with bolus degradation rates greater ( Low-quality = -0.09 ± 0.007; High-quality = -0.04 ± 0.005) with cows on the low-quality diet versus the high-quality diet. Across both studies, two of four bolus prototypes met target release rates at 90 days. However, bolus prototype degradation characteristics varied and were influenced by diet quality.
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