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Item Glacial ice cores: A model system for developing extraterrestrial decontamination protocols(2005-04) Christner, Brent C.; Mikucki, Jill A.; Foreman, Christine M.; Denson, Jackie; Priscu, John C.Evidence gathered from spacecraft orbiting Mars has shown that water ice exists at both poles and may form a large subsurface reservoir at lower latitudes. The recent exploration of the martian surface by unmanned landers and surface rovers, and the planned missions to eventually return samples to Earth have raised concerns regarding both forward and back contamination. Methods to search for life in these icy environments and adequate protocols to prevent contamination can be tested with earthly analogues. Studies of ice cores on Earth have established past climate changes and geological events, both globally and regionally, but only recently have these results been correlated with the biological materials (i.e., plant fragments, seeds, pollen grains, fungal spores, and microorganisms) that are entrapped and preserved within the ice. The inclusion of biology into ice coring research brings with it a whole new approach towards decontamination. Our investigations on ice from the Vostok core (Antarctica) have shown that the outer portion of the cores have up to 3 and 2 orders of magnitude higher bacterial density and dissolved organic carbon (DOC) than the inner portion of the cores, respectively, as a result of drilling and handling. The extreme gradients that exist between the outer and inner portion of these samples make contamination a very relevant aspect of geomicrobiological investigations with ice cores, particularly when the actual numbers of ambient bacterial cells are low. To address this issue and the inherent concern it raises for the integrity of future investigations with ice core materials from terrestrial and extraterrestrial environments, we employed a procedure to monitor the decontamination process in which ice core surfaces are painted with a solution containing a tracer microorganism, plasmid DNA, and fluorescent dye before sampling. Using this approach, a simple and direct method is proposed to verify the authenticity of geomicrobiological results obtained from ice core materials. Our protocol has important implications for the design of life detection experiments on Mars and the decontamination of samples that will eventually be returned to Earth.Item Biological materials in ice cores(2006) Priscu, John C.; Christner, Brent C.; Foreman, Christine M.; Royston-Bishop, GeorgeItem Limnological conditions in subglacial Lake Vostok, Antarctica(2006-11) Christner, Brent C.; Royston-Bishop, George; Foreman, Christine M.; Arnold, Brianna R.; Tranter, Martyn; Welch, Kathleen A.; Lyons, W. Berry; Tsapin, Alexandre I.; Studinger, Michael; Priscu, John C.Subglacial Lake Vostok is located ~4 km beneath the surface of the East Antarctic Ice Sheet and has been isolated from the atmosphere for >15 million yr. Concerns for environmental protection have prevented direct sampling of the lake water thus far. However, an ice core has been retrieved from above the lake in which the bottom ~85 m represents lake water that has accreted (i.e., frozen) to the bottom of the ice sheet. We measured selected constituents within the accretion ice core to predict geomicrobiological conditions within the surface waters of the lake. Bacterial density is two- to sevenfold higher in accretion ice than the overlying glacial ice, implying that Lake Vostok is a source of bacterial carbon beneath the ice sheet. Phylogenetic analysis of amplified small subunit ribosomal ribonucleic acid (rRNA) gene sequences in accretion ice formed over a deep portion of the lake revealed phylotypes that classify within the β-, y-, and δ-Proteobacteria. Cellular, major ion, and dissolved organic carbon levels all decreased with depth in the accretion ice (depth is a proxy for increasing distance from the shoreline), implying a greater potential for biological activity in the shallow shoreline waters of the lake. Although the exact nature of the biology within Lake Vostok awaits direct sampling of the lake water, our data from the accretion ice support the working hypothesis that a sustained microbial ecosystem is present in this subglacial lake environment, despite high pressure, constant cold, low nutrient input, potentially high oxygen concentrations, and an absence of sunlight.Item Ubiquity of biological ice nucleators in snowfall(2008-02) Christner, Brent C.; Morris, Cindy E.; Foreman, Christine M.; Cai, Rongman; Sands, David C.Despite the integral role of ice nucleators (IN) in atmospheric processes leading to precipitation, their sources and distributions have not been well established. We examined IN in snowfall from mid- and high-latitude locations and found that the most active were biological in origin. Of the IN larger than 0.2 micrometer that were active at temperatures warmer than -7°C, 69 to 100% were biological, and a substantial fraction were bacteria. Our results indicate that the biosphere is a source of highly active IN and suggest that these biological particles may affect the precipitation cycle and/or their own precipitation during atmospheric transport.Item Geographic, seasonal, and precipitation chemistry influence on the abundance and activity of biological ice nucleators in rain and snow(2008-11) Christner, Brent C.; Cai, Rongman; Morris, Cindy E.; McCarter, Kevin S.; Foreman, Christine M.; Skidmore, Mark L.; Montross, Scott N.; Sands, David C.Biological ice nucleators (IN) function as catalysts for freezing at relatively warm temperatures (warmer than −10 °C). We examined the concentration (per volume of liquid) and nature of IN in precipitation collected from Montana and Louisiana, the Alps and Pyrenees (France), Ross Island (Antarctica), and Yukon (Canada). The temperature of detectable ice-nucleating activity for more than half of the samples was ≥ −5 °C based on immersion freezing testing. Digestion of the samples with lysozyme (i.e., to hydrolyze bacterial cell walls) led to reductions in the frequency of freezing (0–100%); heat treatment greatly reduced (95% average) or completely eliminated ice nucleation at the measured conditions in every sample. These behaviors were consistent with the activity being bacterial and/or proteinaceous in origin. Statistical analysis revealed seasonal similarities between warm-temperature ice-nucleating activities in snow samples collected over 7 months in Montana. Multiple regression was used to construct models with biogeochemical data [major ions, total organic carbon (TOC), particle, and cell concentration] that were accurate in predicting the concentration of microbial cells and biological IN in precipitation based on the concentration of TOC, Ca2+, and NH4+, or TOC, cells, Ca2+, NH4+, K+, PO43−, SO42−, Cl−, and HCO3−. Our results indicate that biological IN are ubiquitous in precipitation and that for some geographic locations the activity and concentration of these particles is related to the season and precipitation chemistry. Thus, our research suggests that biological IN are widespread in the atmosphere and may affect meteorological processes that lead to precipitation.Item Bacteria in subglacial environments(2008) Christner, Brent C.; Skidmore, Mark L.; Priscu, John C.; Tranter, Martyn; Foreman, Christine M.Glaciers exist where the annual temperature remains cold enough to allow snowfall to accumulate for an extended period of time and where conditions allow subsequent metamorphosis to ice. Glacial ice forms expansive continental ice sheets in the polar regions, (e.g., in Antarctica and Greenland), and at lower latitudes, ice fields (valley or alpine glaciers) and ice caps (if a volcano or mountain range is completely glaciated) exist globally at high altitude. Temperate glaciers comprise <4% of the glacial ice on the planet, but are important freshwater reservoirs and are often the sources for major rivers vital for irrigation, industry, and providing millions of people with drinking water. The Greenland and Antarctic ice sheets currently cover ~10% of the terrestrial surface (>1.5×107 km2) and contain ~75% of the freshwater on Earth (Paterson 1994). The Antarctic ice sheet alone contains ~90% of the planet's ice and, if melted, would result in a sea level rise of ~65 m (The National Snow and Ice Data Center; http://nsidc.org/).Item Antarctic subglacial water: Origin, evolution and ecology(2008-09) Priscu, John C.; Tulaczyk, Slawek; Studinger, Michael; Kennicutt II, Mahlon C.; Christner, Brent C.; Foreman, Christine M.Recent discoveries in the polar regions have revealed that subglacial environments provide a habitat for life in a setting that was previously thought to be inhospitable. These habitats consist of large lakes, intermittently flowing rivers, wetlands, and subglacial aquifers. This chapter presents an overview of the geophysical, chemical, and biological properties of selected subglacial environments. The focus is on the large subglacial systems lying beneath Antarctic ice sheets where most of the subglacial water on our planet is thought to exist. Specifically, this chapter addresses the following topics: (1) the distribution, origin, and hydrology of Antarctic subglacial lakes; (2) Antarctic ice streams as regions of dynamic liquid-water movement that influence ice-sheet dynamics; and (3) subglacial environments as habitats for life and reservoirs of organic carbon.Item Recrystallization inhibition in ice due to ice binding protein activity detected by nuclear magnetic resonance(2014-09) Brown, Jennifer R.; Seymour, Joseph D.; Brox, T. I.; Skidmore, Mark L.; Wang, Chen; Christner, Brent C.; Luo, B. H.; Codd, Sarah L.Liquid water present in polycrystalline ice at the interstices between ice crystals results in a network of liquid-filled veins and nodes within a solid ice matrix, making ice a low porosity porous media. Here we used nuclear magnetic resonance (NMR) relaxation and time dependent self-diffusion measurements developed for porous media applications to monitor three dimensional changes to the vein network in ices with and without a bacterial ice binding protein (IBP). Shorter effective diffusion distances were detected as a function of increased irreversible ice binding activity, indicating inhibition of ice recrystallization and persistent small crystal structure. The modification of ice structure by the IBP demonstrates a potential mechanism for the microorganism to enhance survivability in ice. These results highlight the potential of NMR techniques in evaluation of the impact of IBPs on vein network structure and recrystallization processes; information useful for continued development of ice-interacting proteins for biotechnology applications.