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Item Evaluation of the effectiveness of entomopathogens for the management of wireworms (Coleoptera: Elateridae) on spring wheat(2014-07) Reddy, Gadi V. P.; Tangtrakulwanich, Khanobporn; Wu, Shaohui; Miller, John H.; Ophus, Victoria L.; Prewett, Julie; Jaronski, Stefan T.Wireworms, the larval stage of click beetles (Coleoptera: Elateridae), are serious soil dwelling pests of small grains, corn, sugar beets, and potatoes. Limonius californicus and Hypnoidus bicolor are the predominant wireworm species infesting wheat in Montana, particularly in the ‘Golden Triangle’ area of north-central Montana. Wireworm populations in field crops are increasing, but currently available insecticides provide only partial control, and no alternative management tools exist. In our study, three entomopathogenic fungi were tested for their efficacy against wireworms in spring wheat at two field locations (Ledger and Conrad, Montana, USA) in 2013. The three fungi (Metarhizium brunneum F52, Beauveria bassiana GHA, and Metarhizium robertsii DWR 346) were evaluated as seed-coat, in-furrow granular, and soil band-over-row drench applications in addition to imidacloprid (Gaucho® 600) seed treatment (as a chemical check), the approach currently being used by growers. Wireworm damage in these treatments was evaluated as standing plant counts, wireworm population surveys, and yield. The three fungi, applied as formulated granules or soil drenches, and the imidacloprid seed treatment all resulted in significantly higher plant stand counts and yields at both locations than the fungus-coated seed treatments or the untreated control. Significant differences were detected among the application methods but not among the species of fungi within each application method. All three fungi, when applied as granules in furrow or as soil drenches, were more effective than when used as seed-coating treatments for wireworm control, and provided an efficacy comparable or superior to imidacloprid. The fungi used in this study provided significant plant and yield protection under moderate wireworm pressure, supporting their value in the management of this pest.Item Photoperiod and Temperature Effects on the Adult Eclosion and Mating Rhythms in Pseudopidorus fasciata (Lepidoptera: Zygaenidae)(2014-12) Wu, Shaohui; Refinetti, Roberto; Kok, Loke T.; Youngman, Roger R.; Reddy, Gadi V. P.; Xue, Fang-SenDaily distributions of eclosion and mating activities of Pseudopidorus fasciata Walker (Lepidoptera: Zygaenidae) were recorded under natural and various laboratory conditions. Eclosion of this insect exhibited circadian gating in constant darkness (DD) but not in constant light (LL) at 28°C. Under natural conditions, the majority of adults emerged in midmorning with an eclosion peak around 1000 hours. The eclosion distribution was significantly affected by ambient temperature but not by photoperiod under laboratory conditions. Eclosion was more spread out at 22°C than at higher temperatures, and peak eclosion times were advanced at higher temperatures up to 30°C. Under natural and laboratory diurnal cycles, adults of P. fasciata preferred to mate at dusk, within a few hours before the start of the scotophase. Photoperiod and ambient temperature interacted in regulating the mating distribution in P. fasciata. Mating rhythmicity disappeared under DD and LL, under which the insect either mated arrhythmically (DD) or barely mated (LL). Overall, eclosion rhythm in this insect was predominantly regulated by temperature rather than photoperiod, whereas photoperiod appeared to be more influential than temperature in rhythmic gate of mating patterns.