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Item Serum mineral concentrations in weaned Montana ram lambs and effects of dietary zinc source and concentration on developing Targhee rams(Montana State University - Bozeman, College of Agriculture, 2017) Page, Chad McBride; Chairperson, Graduate Committee: Tom Murphy; T. W. Murphy, M. L. Van Emon, J. G. P. Bowman, S. A. Wyffels and W. C. Stewart were co-authors of the article, 'Survey of serum trace mineral concentration in weaned Montana ram lambs: implications for trace mineral supplementation in weaned rams' submitted to the journal 'Journal of animal science' which is contained within this thesis.; M. L. Van Emon, T. W. Murphy, C. K. Larson, J. G. Berardinelli, I. R. McGregor and W. C. Stewart were co-authors of the article, 'Effects of zinc source and dietary concentration on zinc status, growth performance, wool traits, and reproductive characteristics in developing rams' submitted to the journal 'Journal of animal science' which is contained within this thesis.Trace mineral deficiencies exist in livestock operations and can contribute to decreased productivity and profitability. The objective of the first study was to quantify serum trace mineral concentrations in weaned ram lambs, with particular emphasis on Se and Zn. Serum samples (n = 221) were collected from ram lambs at 21 ranches throughout Montana and analyzed for Co, Cu, Fe, Mn, Mo, Se, and Zn concentrations. Additionally, water samples were analyzed for pertinent characteristics. Of ranches surveyed, only 67% provided a complete mineral supplement. Sheep that were provided supplementary trace mineral had greater serum Se concentrations (P < 0.001). Based on serum trace mineral concentration reference ranges, the two most commonly deficient and marginally deficient minerals across Montana were Se (19 and 23.8% of ranches, respectively) and Zn (9.5 and 57.1%, respectively). Of ranches sampled, 40 and 35% of water samples exceeded excessive concentrations in Na and sulfates, respectively. This regional knowledge of serum trace mineral concentrations in a sample of ram lambs can provide information for ranches to evaluate current and future mineral supplementation needs, as well as aid the feed industry in designing formulations for mineral premixes. Based on these results, a second study was designed with the objective to evaluate the effects of dietary Zn source and concentration on serum Zn status, growth performance, wool traits, and reproductive characteristics of developing yearling rams. Forty-four Targhee rams were used in an 84-d completely randomized design and were fed one of three pelleted dietary treatments: 1) a control diet without fortified Zn; 2) a diet fortified with a Zn amino acid complex (ZnAA); and 3) a diet fortified with ZnSO4. Serum samples were collected from each ram at four time periods to quantify serum Zn and testosterone concentrations. ZnSO4 had greater (P < or = 0.03) serum Zn concentrations compared to other treatments. Rams consuming ZnAA had greater (P < or = 0.03) average daily gain than rams in the other treatments. Wool regrowth was greater (P < 0.001) in the ZnSO4 treatment group compared to control. These results indicate that source and concentration of a Zn supplement appears to affect ram development.Item Effects of ewe late gestational supplementation of rumen undegradable protein, vitamin E, zinc, and chlortetracycline on ewe productivity and postweaning management of lambs on feedlot performance and tissue deposition(Montana State University - Bozeman, College of Agriculture, 2009) Redden, Roy Reid, 1981-; Chairperson, Graduate Committee: Patrick G. Hatfield.Lamb survival and productivity from birth to weaning and lamb postweaning management harvest are areas that the US sheep industry needs to become more efficient at to remain profitable. Western white-faced ewes were supplemented HIGH (12.5% rumen by-pass protein, 880 IU/kg of supplemental Vitamin E, 176 ppm chelated Zn, and 72.7 mg/kg chlortetracycline) or LOW (7.56% rumen by-pass protein, with no supplemental Vitamin E, chelated Zn, or chlortetracycline) supplements at 0.227 kg·ewe -1·d -1 during late gestation. Ewes of different age and body condition scores were individually supplemented for 29 d prior to expected lambing. Thereafter, each ewe was mass fed the appropriate supplement until lambing. In Experiment 3, approximately 600 ewes were group fed HIGH or LOW supplements over 2 yr. Differences in antibody transfer from ewe to lamb were detected in supplemented ewes of different age (P < 0.10); however, lamb production was not different (P > 0.10) for all 3 experiments. To investigate lamb post-weaning management, terminally sired lambs (n = 72) were randomly assigned to 1 of 4 backgrounding treatments. Lamb backgrounding treatments were: ad libitum access to 80% alfalfa: 20% barley pellets (PELLET); cool season grass paddock grazing (GRASS); remain with ewe flock on fall dormant range (LATE WEAN); wean for 96 h and returned to ewe flock on dormant range (RANGE). Background treatments were applied for 29 d. Thereafter, lambs were finished on a corn based diet. Lamb BW and ultrasound measurements were taken at weaning, after background treatment, after feedlot step-up and at the conclusion of the finishing period. Pen intake was measured. Lambs backgrounded on PELLET were heavier (P < 0.10) than all other treatments after the backgrounding period and at the end of the feedlot period. Lambs backgrounded on PELLET had the greatest intakes and ADG (P < 0.10) during the feedlot period. At beginning and end of the feedlot period, PELLET and GRASS lambs had larger (P < 0.05) LM areas than RANGE and LATE WEAN treated lambs. Under the condition of the studies, late gestational supplements did not improve ewe productivity and backgrounding treatments on dormant range diminished feedlot productivity.Item Investigation of the effects of copper source, copper and zinc levels, and dietary protein source on CU bioavialability in rainbow trout(Montana State University - Bozeman, College of Agriculture, 2012) Read, Elizabeth Stevens; Chairperson, Graduate Committee: John Paterson.Limited research has examined the effects that plant-based diets have on copper (Cu) and Zinc (Zn) absorption and utilization in rainbow trout. Few studies have been conducted to determine if interactions exist in the utilization of Cu when increasing levels of supplementary Zn were offered. The objectives of this research were to: first determine what effect protein source (plant vs. animal based), Cu source (complex vs. inorganic) and concentrations of Cu (0, 5, 10, 15, 20 ppm) in the diet had on rate and efficiency of gain and Cu tissue levels in rainbow trout. The second experiment was to determine if interactions occur due to increasing diet content of Zn (0, 30, 300, 1500 ppm) and Cu provided at two levels (0 or 10 ppm) on tissue levels of Cu in rainbow trout. From experiment one, trout fed plant-based diet had higher (P < 0.05) ADG and improved (P < 0.05) FCR in comparison to fishmeal fed trout. Highest (P < 0.05) hepatic Cu concentrations were also observed in trout fed plant-based diets. No differences (P >0.05) were observed in growth or hepatic concentrations due to Cu source. From experiment two, no antagonistic interactions were observed between increasing levels of dietary Zn and Cu. Trout fed the two highest levels of dietary Zn (300 and 1500 ppm) had the greatest (P < 0.05) weight gains. Dietary Zn supplementation increased (P < 0.05) whole body Cu at 12 wks. With increasing dietary Zn supplementation, resulted in increased (P < 0.05) whole body Zn. Cataracts and tail rot were observed at 12 wks in trout fed the Cu and Zn deficient diet. In conclusion, plant-based diets enhanced Cu bioavailability indicated by higher weight gains and hepatic Cu concentrations in experiment one, compared to trout fed fishmeal-based diets. Cu supplementation is required in a plant-based in order to achieve optimal growth in trout. Results of the second study indicate rainbow trout fed plant-based diets require Zn supplementation to obtain sufficient growth. The highest levels of Zn supplementation did not impair Cu uptake in rainbow trout.