Scholarship & Research
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Item Microbial ecology of mosquitos and ticks(Montana State University - Bozeman, College of Letters & Science, 2017) Pinkham, Nicholas Vernon; Chairperson, Graduate Committee: Seth WalkHost evolutionary history has been shown to select for distinct host associated microbial communities over large evolutionary time scales. The microbiomes of disease vector have been shown to alter the capacity of their host to vector pathogens. Much remains to be understood about how the microbiome of mosquitos and ticks assemble in situ. We conducted a large-scale investigation of microbiome composition between mosquito species as well as a second investigation of microbiomes of brown dog ticks collected in Iquitos, Peru. Intraspecific and interspecific bacterial community diversity was compared across 26 species of mosquitoes collected in Montana. Previous studies of lab reared mosquitoes report greater variation in microbial communities between species than within. Using 16S rRNA sequencing we observed a large amount of intraspecific variation in microbiomes, as well as different species hosting very similar microbiomes. The tick microbiome was found to be dominated by a few select community members that were seen at an extremely high abundance and resembled intracellular tick-borne pathogens. It is common for ticks to host endosymbionts that closely to human pathogens but are not pathogenic themselves. Negative interactions were seen between the most abundant organism observed in the ticks.Item The role of SaeR/S in secondary Staphylococcus aureus Pneumonia(Montana State University - Bozeman, College of Agriculture, 2016) Hisey, Bennett Stephen; Chairperson, Graduate Committee: Jovanka Voyich-Kane; Timothy R Borgogna, Kyler B. Pallister, Eli W. Sward, Fermin E. Guerra and Jovanka M Voyich were co-authors of the article, 'The role of SaeR/S in secondary Staphylococcus aureus pneumonia' submitted to the journal 'Journal of infectious diseases' which is contained within this thesis.Methicillin?resistant Staphylococcus aureus (MRSA) is a Gram?positive pathogen capable of causing diverse disease in humans. MRSA precisely controls virulence factor expression via the SaeR/S two?component gene regulatory system. While much is known about SaeR/S regulation patterns during skin infection, less is understood about the role it plays in the pulmonary environment during secondary staphylococcal pneumonia. Using an isogenic deletion mutant in pulsed field gel electrophoresis type USA300 (strain LAC) of the saeR/S two?component gene regulatory system we examined its role in mouse models of pathogenesis involving primary infection with influenza strain A/WSN/33 followed by USA300 infection. Results demonstrate SaeR/S contributes significantly to mortality during pneumonia following influenza A infection. Reverse transcription PCR and QuantiGene 2.0 assays revealed differences in both transcription of components of SaeR/S as well as virulence factors under SaeR/S control. Primary Influenza infection was seen to up regulate expression of virulence factors under control due to antecedent influenza A infection. These data underscore the importance of pathogen contribution to the pathogenesis of secondary pneumonia.Item Partial characterization of an entomopoxvirus isolated from grasshoppers(Montana State University - Bozeman, College of Agriculture, 1978) Kussman, Herbert CarlItem Cortisol acetate-induced wasting disease in germfree and conventionally reared mice(Montana State University - Bozeman, College of Agriculture, 1966) Reed, Norman DuaneItem Cultural characteristics of certain pathogenic anaerobes isolated from sheep(Montana State University - Bozeman, College of Agriculture, 1935) Chaddock, Theodore ThomasItem Bacterin-induced wasting in mice(Montana State University - Bozeman, College of Agriculture, 1966) Braaten, Bruce AlbertItem The pathogenesis of ovine mastitis due to Pasteurella Mastidis(Montana State University - Bozeman, College of Agriculture, 1951) Firehammer, Burton D.Item Bacteriological studies of post-thymectomy wasting disease(Montana State University - Bozeman, College of Agriculture, 1969) Cantrell, John LeonardItem The development of infections with Listeria monocytogenes following the ingestion of ponderosa pine needles(Montana State University - Bozeman, College of Agriculture, 1979) Adams, Charlotte JutilaItem Studies on the etiology of the runting syndrome in mice(Montana State University - Bozeman, College of Agriculture, 1966) Safford, John William