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Item Agile collaboration: Citizen science as a transdisciplinary approach to heliophysics(Frontiers Media SA, 2023-04) Ledvina, Vincent; Brandt, Laura; MacDonald, Elizabeth; Frissell, Nathaniel; Anderson, Justin; Chen, Thomas Y.; French, Ryan J.; Mare, Francesca Di; Grover, Andrea; Sigsbee, Kristine; Gallardo-Lacourt, Bea; Lach, Donna; Shaw, Joseph A.; Hunnekuhl, Michael; Kosar, Burcu; Barkhouse, Wayne; Young, Tim; Kedhambadi, Chandresh; Ozturk, Dogacan S.; Claudepierre, Seth G.; Dong, Chuanfei; Witteman, Andy; Kuzub, Jeremy; Sinha, GunjanCitizen science connects scientists with the public to enable discovery, engaging broad audiences across the world. There are many attributes that make citizen science an asset to the field of heliophysics, including agile collaboration. Agility is the extent to which a person, group of people, technology, or project can work efficiently, pivot, and adapt to adversity. Citizen scientists are agile; they are adaptable and responsive. Citizen science projects and their underlying technology platforms are also agile in the software development sense, by utilizing beta testing and short timeframes to pivot in response to community needs. As they capture scientifically valuable data, citizen scientists can bring expertise from other fields to scientific teams. The impact of citizen science projects and communities means citizen scientists are a bridge between scientists and the public, facilitating the exchange of information. These attributes of citizen scientists form the framework of agile collaboration. In this paper, we contextualize agile collaboration primarily for aurora chasers, a group of citizen scientists actively engaged in projects and independent data gathering. Nevertheless, these insights scale across other domains and projects. Citizen science is an emerging yet proven way of enhancing the current research landscape. To tackle the next-generation’s biggest research problems, agile collaboration with citizen scientists will become necessary.Item An Aurora B-RPA signaling axis secures chromosome segregation fidelity(Springer Science and Business Media LLC, 2023-05) Roshan, Poonam; Kuppa, Sahiti; Mattice, Jenna R.; Kaushik, Vikas; Chadda, Rahul; Pokhrel, Nilisha; Tumala, Brunda R.; Biswas, Aparna; Bothner, Brian; Antony, Edwin; Origanti, SofiaErrors in chromosome segregation underlie genomic instability associated with cancers. Resolution of replication and recombination intermediates and protection of vulnerable single-stranded DNA (ssDNA) intermediates during mitotic progression requires the ssDNA binding protein Replication Protein A (RPA). However, the mechanisms that regulate RPA specifically during unperturbed mitotic progression are poorly resolved. RPA is a heterotrimer composed of RPA70, RPA32 and RPA14 subunits and is predominantly regulated through hyperphosphorylation of RPA32 in response to DNA damage. Here, we have uncovered a mitosis-specific regulation of RPA by Aurora B kinase. Aurora B phosphorylates Ser-384 in the DNA binding domain B of the large RPA70 subunit and highlights a mode of regulation distinct from RPA32. Disruption of Ser-384 phosphorylation in RPA70 leads to defects in chromosome segregation with loss of viability and a feedback modulation of Aurora B activity. Phosphorylation at Ser-384 remodels the protein interaction domains of RPA. Furthermore, phosphorylation impairs RPA binding to DSS1 that likely suppresses homologous recombination during mitosis by preventing recruitment of DSS1-BRCA2 to exposed ssDNA. We showcase a critical Aurora B-RPA signaling axis in mitosis that is essential for maintaining genomic integrity.