Cryosectioning of biofilms for microscopic examination

dc.contributor.authorYu, Feipeng Philip
dc.contributor.authorCallis, G. M.
dc.contributor.authorStewart, Philip S.
dc.contributor.authorGriebe, Thomas
dc.contributor.authorMcFeters, Gordon A.
dc.date.accessioned2017-12-14T21:32:10Z
dc.date.available2017-12-14T21:32:10Z
dc.date.issued1994
dc.description.abstractA method for rapid and minimally disruptive embedding and sectioning of bacterial biofilms has been developed and applied to binary population biofilms of Klebsiella pneumoniae and Pseudomonas aeruginosa grown on stainless steel surfaces in continuous flow annular reactors. Biofilms were cryoembedded using a commercial tissue embedding medium. Frozen embedded biofilms could be removed easily from the substratum by gently flexing the steel coupon. Microscopic examination of the substratum surface after biofilm removal indicated that less than a monolayer of attached cells remained. Five μm thick frozen sections were cut with a cryostat and examined by light or fluorescence microscopy. The cryoembedding technique preserved biofilm structural features including an irregular surface, water channels, local protrusions up to 500 μm thick, and a well‐defined substratum interface. The method requires minimal sample processing without dehydration or prolonged fixation, and can be completed in less than 24 h.en_US
dc.identifier.citationYu, F.P., G.M. Callis, P.S. Stewart, T. Griebe, and G.A. McFeters, "Cryosectioning of biofilms for microscopic examination," Biofouling, 8:85-91 (1994).en_US
dc.identifier.issn0892-7014
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/14085
dc.titleCryosectioning of biofilms for microscopic examinationen_US
dc.typeArticleen_US
mus.citation.extentfirstpage85en_US
mus.citation.extentlastpage91en_US
mus.citation.issue2en_US
mus.citation.journaltitleBiofoulingen_US
mus.citation.volume8en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US
mus.data.thumbpage4en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1080/08927019409378264en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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