Mutation in the Endo-β-1,4-glucanase (KORRIGAN) Is Responsible for Thick Leaf Phenotype in Sorghum

dc.contributor.authorMendu, Lavanya
dc.contributor.authorJalathge, Gayani
dc.contributor.authorKaur Dhillon, Kamalpreet
dc.contributor.authorPratap Singh, Nagendra
dc.contributor.authorKumar Balasubramanian, Vimal
dc.contributor.authorFewou, Rebecca
dc.contributor.authorGitz, Dennis C.
dc.contributor.authorChen, Junping
dc.contributor.authorXin, Zhanguo
dc.contributor.authorMendu, Venugopal
dc.date.accessioned2023-02-21T19:03:18Z
dc.date.available2023-02-21T19:03:18Z
dc.date.issued2023-12
dc.description.abstractSorghum [Sorghum bicolor (L.) Moench] is an important crop for food, feed, and fuel production. Particularly, sorghum is targeted for cellulosic ethanol production. Extraction of cellulose from cell walls is a key process in cellulosic ethanol production, and understanding the components involved in cellulose synthesis is important for both fundamental and applied research. Despite the significance in the biofuel industry, the genes involved in sorghum cell wall biosynthesis, modification, and degradation have not been characterized. In this study, we have identified and characterized three allelic thick leaf mutants (thl1, thl2, and thl3). Bulked Segregant Analysis sequencing (BSAseq) showed that the causal mutation for the thl phenotype is in endo-1,4-β-glucanase gene (SbKOR1). Consistent with the causal gene function, the thl mutants showed decreased crystalline cellulose content in the stem tissues. The SbKOR1 function was characterized using Arabidopsis endo-1,4-β-glucanase gene mutant (rsw2-1). Complementation of Arabidopsis with SbKOR1 (native Arabidopsis promoter and overexpression by 35S promoter) restored the radial swelling phenotype of rsw2-1 mutant, proving that SbKOR1 functions as endo-1,4-β-glucanase. Overall, the present study has identified and characterized sorghum endo-1,4-β-glucanase gene function, laying the foundation for future research on cell wall biosynthesis and engineering of sorghum for biofuel production.en_US
dc.identifier.citationMendu L, Jalathge G, Dhillon KK, Singh NP, Balasubramanian VK, Fewou R, Gitz DC III, Chen J, Xin Z, Mendu V. Mutation in the Endo-β-1,4-glucanase (KORRIGAN) Is Responsible for Thick Leaf Phenotype in Sorghum. Plants. 2022; 11(24):3531. https://doi.org/10.3390/plants11243531en_US
dc.identifier.issn2223-7747
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/17710
dc.language.isoen_USen_US
dc.publisherMDPI AGen_US
dc.rightscc-byen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectsorghumen_US
dc.subjectcell wallen_US
dc.subjectendo-1,4-β-glucanaseen_US
dc.subjectkorriganen_US
dc.subjectthick leafen_US
dc.subjectSbKORRIGANen_US
dc.subjectbiofuelen_US
dc.titleMutation in the Endo-β-1,4-glucanase (KORRIGAN) Is Responsible for Thick Leaf Phenotype in Sorghumen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage15en_US
mus.citation.issue24en_US
mus.citation.journaltitlePlantsen_US
mus.citation.volume11en_US
mus.identifier.doi10.3390/plants11243531en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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