Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris

dc.contributor.authorLoss, Manisha
dc.contributor.authorThompson, Katherine G.
dc.contributor.authorAgostinho‐Hunt, Alessandra
dc.contributor.authorJames, Garth A.
dc.contributor.authorMongodin, Emmanuel F.
dc.contributor.authorRosenthal, Ian
dc.contributor.authorCheng, Nancy
dc.contributor.authorLeung, Sherry
dc.contributor.authorChien, Anna L.
dc.contributor.authorKang, Sewon
dc.date.accessioned2022-09-20T17:07:03Z
dc.date.available2022-09-20T17:07:03Z
dc.date.issued2020-12
dc.descriptionThis is the peer reviewed version of the following article: [Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris. International Journal of Dermatology 60, 5 p589-596 (2020)], which has been published in final form at https://doi.org/10.1111/ijd.15308. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions: https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html#3.en_US
dc.description.abstractBackground: The ability of C. acnes strains to form biofilms has been correlated with their virulence. Objective: This study examined biofilm and skin microbiota in acne patients in order to understand their role in the development of acne lesions. Methods: Thin sections of punch biopsy specimens of (1) uninflamed comedones, (2) inflammatory lesions, and (3) uninvolved adjacent skin of acne patients were examined. Epiflourescence and confocal laser scanning microscopy were used for biofilm detection, and pyrosequencing with taxonomic classification of 16s rRNA gene amplicons was used for microbiota analysis. Results: Of the 39 skin specimens from patients with mild-moderate acne (n=13) that were studied, 9 (23%) contained biofilm. Among these specimens, biofilm was most frequently detected in comedones (55.6%) and less frequently in inflammatory papules (22.2%) and uninvolved skin (22.2%). Comedones demonstrated the highest mean alpha diversity of all the lesion subtypes. The relative abundance of Staphylococcus was significantly higher in comedones (11.400% ±12.242%) compared to uninvolved skin (0.073% ±0.185%, p=0.024). Conclusions: The microenvironment of the comedone differs from that of inflammatory lesions and unaffected skin. The increased frequency of biofilm in comedones may account for the lack of host inflammatory response to these lesions.en_US
dc.identifier.citationLoss, Manisha, Katherine G. Thompson, Alessandra Agostinho‐Hunt, Garth A. James, Emmanuel F. Mongodin, Ian Rosenthal, Nancy Cheng, Sherry Leung, Anna L. Chien, and Sewon Kang. "Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris." International Journal of Dermatology 60, no. 5 (2021): 589-596.en_US
dc.identifier.issn0011-9059
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/17188
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.rightscopyright Wiley 2020en_US
dc.rights.urihttps://web.archive.org/web/20200106202133/https://onlinelibrary.wiley.com/library-info/products/price-listsen_US
dc.rights.urihttp://web.archive.org/web/20190530141919/https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.htmlen_US
dc.subjectacne biofilmen_US
dc.subjectmicrobiota correlationen_US
dc.titleNoninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgarisen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage25en_US
mus.citation.issue5en_US
mus.citation.journaltitleInternational Journal of Dermatologyen_US
mus.citation.volume60en_US
mus.identifier.doi10.1111/ijd.15308en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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