Jugular Vein Catheterization for Continuous Multi-hour Infusions in Mice

Abstract

In vivo metabolic labeling in animal models is difficult and poorly described in method papers. This is an underutilized technique when it comes to identifying important metabolic components. In this paper we describe a detailed up to date protocol for metabolic administration of metabolites into mice. This protocol is designed to make these procedures more available to the research community. This methodology can be utilized for delivery for other compounds such as drugs and isotopes. The primary focus of this review is to emphasize that jugular catheterization is a versatile and effective way of studying the effects of compounds on the entire body.