The Formation of Migratory Ripples in a Mixed Species Bacterial Biofilm Growing in Turbulent Flow

dc.contributor.authorStoodley, Paul
dc.contributor.authorLewandowski, Zbigniew
dc.contributor.authorBoyle, John D.
dc.contributor.authorLappin-Scott, H. M.
dc.date.accessioned2017-11-03T15:21:42Z
dc.date.available2017-11-03T15:21:42Z
dc.date.issued1999-09
dc.description.abstractMixed-species biofilms, consisting of Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluorescens and Stenotrophomonas maltophilia, were grown in glass flow cells under either laminar or turbulent flow. The biofilms grown in laminar flow consisted of roughly circular-shaped microcolonies separated by water channels. In contrast, biofilm microcolonies grown in turbulent flow were elongated in the downstream direction, forming filamentous ‘streamers’. Moreover, biofilms growing in turbulent flow developed extensive patches of ripple-like structures between 9 and 13 days of growth. Using time-lapse microscopic imaging, we discovered that the biofilm ripples migrated downstream. The morphology and the migration velocity of the ripples varied with short-term changes in the bulk liquid flow velocity. The ripples had a maximum migration velocity of 800 μm h−1 (2.2 × 10−7 m s−1) when the liquid flow velocity was 0.5 m s−1(Reynolds number = 1800). This work challenges the commonly held assumption that biofilm structures remain at the same location on a surface until they eventually detach.en_US
dc.identifier.citationStoodley, P., Z. Lewandowksi, J.D. Boyle, and H.M. Lappin-Scott, "The Formation of Migratory Ripples in a Mixed Species Bacterial Biofilm Growing in Turbulent Flow," Environ. Microbiol. 1(5):447455 (1999).en_US
dc.identifier.issn1462-2912
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/13946
dc.titleThe Formation of Migratory Ripples in a Mixed Species Bacterial Biofilm Growing in Turbulent Flowen_US
dc.typeArticleen_US
mus.citation.extentfirstpage447en_US
mus.citation.extentlastpage455en_US
mus.citation.issue5en_US
mus.citation.journaltitleEnvironmental Microbiologyen_US
mus.citation.volume1en_US
mus.contributor.orcidStoodley, Paul|0000-0001-6069-273Xen_US
mus.data.thumbpage4en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1046/j.1462-2920.1999.00055.xen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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