A collection of archaeal 16S rRNA Clone-FISH cultures for probe validation in fluorescence in situ hybridization experiments

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van beek-archaeal-16s-rrna-clone-fish-cultures-fluorescence-in-situ-hybridization-experiments-2025.pdf (1.52 MB)

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2025-11

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American Society for Microbiology

DOI

10.1128/mra.01131-25

Abstract

We present a collection of 30 Escherichia coli cultures (Clone-FISH cultures), each carrying a plasmid for the heterologous expression of a (near) full-length 16S rRNA gene from 1 of 30 lineages of archaea, including 17 yet uncultured ones. We make these clones available for use as controls in fluorescence in situ hybridization experiments.

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Keywords

archaea , fluorescence in situ hybridization , controls , microscopy , taxonomic identification , plasmids , 16s rRNA , E. coli , CloneFISH , microbial ecology

Citation

Van Beek JM, Robles G, Mewalal R, Blaby I,Hatzenpichler R.2025.A collection of archaeal 16S rRNA Clone-FISH cultures for probe validation in fluorescence in situ hybridization experiments. Microbiol Resour Announc14:e01131-25.https://doi.org/10.1128/mra.01131-25

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https://scholarworks.montana.edu/handle/1/20047

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Scholarly Work - Microbiology & Cell Biology

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Copyright © American Society for Microbiology, [A collection of archaeal 16S rRNA Clone-FISH cultures for probe validation in fluorescence in situ hybridization experiments. Microbiology Resource Announcements 14, 12 pe0113125 (2025)], Find the version of record at https://doi.org/10.1128/mra.01131-25