Stem rust resistance in wheat is suppressedby a subunit of the mediator complex

dc.contributor.authorHiebert, Colin W.
dc.contributor.authorMoscou, Matthew J.
dc.contributor.authorHewitt, Tim
dc.contributor.authorSteuernagel, Burkhard
dc.contributor.authorHernandez-Pinzon, Inma
dc.contributor.authorGreen, Phon
dc.contributor.authorPujol, Vincent
dc.contributor.authorZhang, Peng
dc.contributor.authorRouse, Matthew N.
dc.contributor.authorJin, Yue
dc.contributor.authorMcIntosh, Robert A.
dc.contributor.authorUpadhyaya, Narayana
dc.contributor.authorZhang, Jianping
dc.contributor.authorBhavani, Sridhar
dc.contributor.authorVrana, Jan
dc.contributor.authorKarafiatova, Miroslava
dc.contributor.authorHuang, Li
dc.contributor.authorFetch, Tom
dc.contributor.authorDolezel, Jaroslav
dc.contributor.authorWulff, Brande B. H.
dc.contributor.authorLagudah, Evans
dc.contributor.authorSpielmeyer, Wolfgang
dc.date.accessioned2021-01-04T20:46:04Z
dc.date.available2021-01-04T20:46:04Z
dc.date.issued2020-02
dc.description.abstractStem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.en_US
dc.identifier.citationHiebert, C.W., Moscou, M.J., Hewitt, T. et al. Stem rust resistance in wheat is suppressed by a subunit of the mediator complex. Nat Commun 11, 1123 (2020). DOI: 10.1038/s41467-020-14937-2en_US
dc.identifier.issn2041-1723
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/16092
dc.language.isoen_USen_US
dc.rights© This manuscript version is made available under the CC-BY 4.0 licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleStem rust resistance in wheat is suppressedby a subunit of the mediator complexen_US
dc.typeArticleen_US
mus.citation.journaltitleNature Communicationsen_US
mus.citation.volume11en_US
mus.data.thumbpage2en_US
mus.identifier.doi10.1038/s41467-020-14937-2en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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