Contributions of antibiotic penetration, oxygen limitation, and low metabolic activity to tolerance of Pseudomonas aeruginosa biofilms to ciprofloxacin and tobramycin

dc.contributor.authorWalters, Marshall C., III
dc.contributor.authorRoe, Frank L.
dc.contributor.authorBugnicourt, Amandine
dc.contributor.authorFranklin, Michael J.
dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-07-27T15:50:27Z
dc.date.available2017-07-27T15:50:27Z
dc.date.issued2003-01
dc.description.abstractThe roles of slow antibiotic penetration, oxygen limitation, and low metabolic activity in the tolerance of Pseudomonas aeruginosa in biofilms to killing by antibiotics were investigated in vitro. Tobramycin and ciprofloxacin penetrated biofilms but failed to effectively kill the bacteria. Bacteria in colony biofilms survived prolonged exposure to either 10 micro g of tobramycin ml(-1)or 1.0 micro g of ciprofloxacin ml(-1). After 100 h of antibiotic treatment, during which the colony biofilms were transferred to fresh antibiotic-containing plates every 24 h, the log reduction in viable cell numbers was only 0.49 +/- 0.18 for tobramycin and 1.42 +/- 0.03 for ciprofloxacin. Antibiotic permeation through colony biofilms, indicated by a diffusion cell bioassay, demonstrated that there was no acceleration in bacterial killing once the antibiotics penetrated the biofilms. These results suggested that limited antibiotic diffusion is not the primary protective mechanism for these biofilms. Transmission electron microscopic observations of antibiotic-affected cells showed lysed, vacuolated, and elongated cells exclusively near the air interface in antibiotic-treated biofilms, suggesting a role for oxygen limitation in protecting biofilm bacteria from antibiotics. To test this hypothesis, a microelectrode analysis was performed. The results demonstrated that oxygen penetrated 50 to 90 micro m into the biofilm from the air interface. This oxic zone correlated to the region of the biofilm where an inducible green fluorescent protein was expressed, indicating that this was the active zone of bacterial metabolic activity. These results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.en_US
dc.identifier.citationWalters, MC 3rd, Roe F, Bugnicourt A, Franklin MJ, Stewart PS, "Contributions of antibiotic penetration, oxygen limitation, and low metabolic activity to tolerance of Pseudomonas aeruginosa biofilms to ciprofloxacin and tobramycin," Antimicrob Agents Chemother, 2003 47(1):317-323en_US
dc.identifier.issn0066-4804
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/13430
dc.titleContributions of antibiotic penetration, oxygen limitation, and low metabolic activity to tolerance of Pseudomonas aeruginosa biofilms to ciprofloxacin and tobramycinen_US
dc.typeArticleen_US
mus.citation.extentfirstpage317en_US
mus.citation.extentlastpage323en_US
mus.citation.issue1en_US
mus.citation.journaltitleAntimicrobial Agents and Chemotherapyen_US
mus.citation.volume47en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US
mus.data.thumbpage6en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aac.47.1.317-323.2003en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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