Laser desorption VUV postionization MS imaging of a cocultured biofilm

dc.contributor.authorBhardwaj, C.
dc.contributor.authorMoore, J. F.
dc.contributor.authorCui, Y.
dc.contributor.authorGasper, G. L.
dc.contributor.authorBerstein, H. C.
dc.contributor.authorCarlson, Ross P.
dc.contributor.authorHanley, L.
dc.date.accessioned2017-01-27T20:35:26Z
dc.date.available2017-01-27T20:35:26Z
dc.date.issued2013-09
dc.description.abstractLaser desorption postionization mass spectrometry (LDPI-MS) imaging is demonstrated with a 10.5 eV photon energy source for analysis and imaging of small endogenous molecules within intact biofilms. Biofilm consortia comprised of a synthetic Escherichia coli K12 coculture engineered for syntrophic metabolite exchange are grown on membranes and then used to test LDPI-MS analysis and imaging. Both E. coli strains displayed many similar peaks in LDPI-MS up to m/z 650, although some observed differences in peak intensities were consistent with the appearance of byproducts preferentially expressed by one strain. The relatively low mass resolution and accuracy of this specific LDPI-MS instrument prevented definitive assignment of species to peaks, but strategies are discussed to overcome this shortcoming. The results are also discussed in terms of desorption and ionization issues related to the use of 10.5 eV single-photon ionization, with control experiments providing additional mechanistic information. Finally, 10.5 eV LDPI-MS was able to collect ion images from intact, electrically insulating biofilms at ∼100 μm spatial resolution. Spatial resolution of ∼20 μm was possible, although a relatively long acquisition time resulted from the 10 Hz repetition rate of the single-photon ionization source.en_US
dc.identifier.citationBhardwaj C, Moore JF, Cui Y, Gasper GL, Bernstein HC, Carlson RP, Hanley L, "Laser desorption VUV postionization MS imaging of a cocultured biofilm," Analytical and Bioanalytical Chemistry. September 2013 405(22):6969-77en_US
dc.identifier.issn1618-2642
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/12449
dc.titleLaser desorption VUV postionization MS imaging of a cocultured biofilmen_US
dc.typeArticleen_US
mus.citation.extentfirstpage6969en_US
mus.citation.extentlastpage6977en_US
mus.citation.issue22en_US
mus.citation.journaltitleAnalytical and Bioanalytical Chemistryen_US
mus.citation.volume405en_US
mus.data.thumbpage7en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1007/s00216-012-6454-0en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentElectrical & Computer Engineering.en_US
mus.relation.departmentMathematical Sciences.en_US
mus.relation.departmentMechanical & Industrial Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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