Spatial distribution and coexistence of klebsiella pneumoniae and pseudomonas aeruginosa in biofilms

dc.contributor.authorStewart, Philip S.
dc.contributor.authorCamper, Anne K.
dc.contributor.authorHandran, S. D.
dc.contributor.authorHuang, Ching-Tsan
dc.contributor.authorWarnecke, Malcolm R.
dc.date.accessioned2018-02-01T22:38:35Z
dc.date.available2018-02-01T22:38:35Z
dc.date.issued1997-01
dc.description.abstractThe heterotrophic bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa stably coexisted in laboratory-grown biofilms, even though the growth rate of K. pneumoniae was twice that of P. aeruginosa under planktonic growth conditions. The failure of K. pneumoniae to displace P. aeruginosa from the biofilm could not be attributed to concentration gradients of the limiting nutrient (glucose) arising from the interaction of reaction and diffusion. Comparisons of the growth rates of the two species in mono- and binary-population biofilms suggested partial segregation of the two species in the latter. We used a fluorescently labeled monoclonal antibody to examine the spatial distribution of K. pneumoniae in frozen cross sections of biofilm to confirm this segregation. K. pneumoniae microcolonies resided on top of, or intermixed with, a base film of P. aeruginosa. We hypothesize that microscale structural heterogeneity and differing rates of bacterial attachment and detachment of the two species are responsible for coexistence in this system.en_US
dc.identifier.citationSpatial distribution and coexistence of klebsiella pneumoniae and pseudomonas aeruginosa in biofilmsen_US
dc.identifier.issn0095-3628
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/14289
dc.titleSpatial distribution and coexistence of klebsiella pneumoniae and pseudomonas aeruginosa in biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage2en_US
mus.citation.extentlastpage10en_US
mus.citation.issue1en_US
mus.citation.journaltitleMicrobial Ecologyen_US
mus.citation.volume33en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US
mus.data.thumbpage7en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/s002489900002en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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