Identification and Characterization of Bacteria-Derived Antibiotics for the Biological Control of Pea Aphanomyces Root Rot

dc.contributor.authorLai, Xiao
dc.contributor.authorNiroula, Dhirendra
dc.contributor.authorBurrows, Mary
dc.contributor.authorWu, Xiaogang
dc.contributor.authorYan, Qing
dc.date.accessioned2023-11-09T23:34:06Z
dc.date.available2023-11-09T23:34:06Z
dc.date.issued2022-08
dc.description.abstractAntibiosis has been proposed to contribute to the beneficial bacteria-mediated biocontrol against pea Aphanomyces root rot caused by the oomycete pathogen Aphanomyces euteiches. However, the antibiotics required for disease suppression remain unknown. In this study, we found that the wild type strains of Pseudomonas protegens Pf-5 and Pseudomonas fluorescens 2P24, but not their mutants that lack 2,4-diacetylphloroglucinol, strongly inhibited A. euteiches on culture plates. Purified 2,4-diacetylphloroglucinol compound caused extensive hyphal branching and stunted hyphal growth of A. euteiches. Using a GFP-based transcriptional reporter assay, we found that expression of the 2,4-diacetylphloroglucinol biosynthesis gene phlAPf-5 is activated by germinating pea seeds. The 2,4-diacetylphloroglucinol producing Pf-5 derivative, but not its 2,4-diacetylphloroglucinol non-producing mutant, reduced disease severity caused by A. euteiches on pea plants in greenhouse conditions. This is the first report that 2,4-diacetylphloroglucinol produced by strains of Pseudomonas species plays an important role in the biocontrol of pea Aphanomyces root rot.en_US
dc.identifier.citationLai X, Niroula D, Burrows M, Wu X, Yan Q. Identification and Characterization of Bacteria-Derived Antibiotics for the Biological Control of Pea Aphanomyces Root Rot. Microorganisms. 2022; 10(8):1596. https://doi.org/10.3390/microorganisms10081596en_US
dc.identifier.issn2076-2607
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/18195
dc.language.isoen_USen_US
dc.publisherMDPI AGen_US
dc.rightscc-byen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectantibioticsen_US
dc.subject2,4-DAPGen_US
dc.subjectbiocontrolen_US
dc.subjectPseudomonas sppen_US
dc.subjectAphanomyces euteichesen_US
dc.titleIdentification and Characterization of Bacteria-Derived Antibiotics for the Biological Control of Pea Aphanomyces Root Roten_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage15en_US
mus.citation.issue8en_US
mus.citation.journaltitleMicroorganismsen_US
mus.citation.volume10en_US
mus.data.thumbpage6en_US
mus.identifier.doi10.3390/microorganisms10081596en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
lai-root-2022.pdf
Size:
3.55 MB
Format:
Adobe Portable Document Format
Description:
pea root rot
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Copyright (c) 2002-2022, LYRASIS. All rights reserved.