A microtiter-plate screening method for biofilm disinfection and removal

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dc.contributor.authorPitts, Betsey
dc.contributor.authorHamilton, Martin A.
dc.contributor.authorZelver, Nick
dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-08-11T20:32:51Z
dc.date.available2017-08-11T20:32:51Z
dc.date.issued2003-08
dc.description.abstractA quantitative spectrophotometric method was developed to measure the removal and killing efficacy of antibiofilm agents. Biofilms of Pseudomonas aeruginosa or Staphylococcus epidermidis were grown in 96-well plates, treated with an agent, then stained with either the biomass indicator crystal violet or the respiratory indicator 5-cyano-2,3-ditolyl tetrazolium chloride. This rapid screening method is sensitive enough to elucidate concentration–response relationships as well as differences between species responses to treatments. Using these assays, agents can be ranked by their ability to remove or kill biofilm.en_US
dc.identifier.citationPitts, B., M.A. Hamilton, N. Zelver, and P.S Stewart, "A microtiter-plate screening method for biofilm disinfection and removal," J. Microbiol. Methods, 54(2):269-276 (2003).en_US
dc.identifier.issn0167-7012
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/13496
dc.titleA microtiter-plate screening method for biofilm disinfection and removalen_US
dc.typeArticleen_US
mus.citation.extentfirstpage269en_US
mus.citation.extentlastpage276en_US
mus.citation.issue2en_US
mus.citation.journaltitleJournal of Microbiological Methodsen_US
mus.citation.volume54en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US
mus.data.thumbpage5en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1016/s0167-7012(03)00034-4en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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