Hunting for Extremophiles: A Systematic Screening of Freshwater Microalgae for Tolerance to High-pH and High-Alkalinity Cultivation

Abstract

Microalgae hold the potential to supply sustainable food, fuel, plastics, and chemicals at commercial scales. Cultivating microalgae at extreme pH (>10) and high alkalinity provides multiple benefits, including (1) reducing the risk of contamination by undesired organisms and (2) enabling direct air capture of CO2, which expands the land area suitable for algae farming compared to using CO2 point sources alone. However, we currently have a limited understanding of which algal taxa can grow under these conditions. Therefore, we conducted a high-throughput screening of 49 freshwater microalgae strains, comprising 40 species, for their ability to grow in moderate (pH 8.5, 25 mM alkalinity), high (pH 10, 75 mM alkalinity), and extreme (pH 10, 150 mM alkalinity) cultivation environments. Our results show that moderate alkalinity tends to significantly increase algae growth (including potentially harmful strains). However, higher levels inhibited all but a small subset of green algae and cyanobacteria. Effects of salinity and alkalinity differed, indicating that they are broadly decoupled. Our results identify new industrially relevant alkaline-tolerant strains, show that algae isolated from “normal” ecosystems can be extremophilic, and suggest that future bioprospecting efforts for alkaline-tolerant algae adapted to local climatic conditions could yield additional productivity gains for the algae industry.