Testing wound dressings using an in vitro wound model
dc.contributor.author | Lipp, C. | |
dc.contributor.author | Kirker, Kelly R. | |
dc.contributor.author | Agostinho, Alessandra | |
dc.contributor.author | James, Garth A. | |
dc.contributor.author | Stewart, Philip S. | |
dc.date.accessioned | 2017-04-11T19:40:49Z | |
dc.date.available | 2017-04-11T19:40:49Z | |
dc.date.issued | 2010-06 | |
dc.description.abstract | Objective: To determine whether or not there are any significant differences in the effects of wound dressings on bacterial bioburden. Method: A selection of non-occlusive, non-adhesive dressings was tested for their effect on bacterial bioburden. The dressings selected included two dressings with antimicrobial properties (one containing silver and one containing PHMB), a cotton-based dressing enclosed in a perforated sleeve of poly(ethylene terephthalate), a carboxymethyl cellulose-based dressing, a fiber-free alginate dressing, and a 12-ply 100% cotton gauze. Using the colony-drip flow reactor (DFR) model, a meticillin-resistant Staphylococcus aureus (MRSA) or Pseudomonas aeruginosa biofilm was grown underneath a dressing sample. Biofilm growth was examined via plate counts, fluorescent microscopy and scanning electron microscopy. Results: The dressings containing antimicrobial agents had the greatest effect on bacterial load. In the MRSA experiments, both antimicrobial dressings produced lower bacteria counts than the other dressings (p<0.001), while in the P. aeruginosa experiments, only the silver-containing sample had fewer bacteria (p<0.0001). However, neither antimicrobial dressing was able to completely eradicate the bacteria when testing with either microorganism. Conclusion: The results presented herein illustrate that bacteria can grow unchallenged within the dressing environment and that an antimicrobial dressing can limit this bacterial growth. Declaration of interest: None. | en_US |
dc.identifier.citation | Lipp C, Kirker K, Agostinho A, James G, Stewart PS, "Testing wound dressings using an in vitro wound model," J Wound Care, 2010 19(6):220-226. | en_US |
dc.identifier.issn | 0969-0700 | |
dc.identifier.uri | https://scholarworks.montana.edu/handle/1/12694 | |
dc.title | Testing wound dressings using an in vitro wound model | en_US |
dc.type | Article | en_US |
mus.citation.extentfirstpage | 220 | en_US |
mus.citation.extentlastpage | 226 | en_US |
mus.citation.issue | 6 | en_US |
mus.citation.journaltitle | Journal of Wound Care | en_US |
mus.citation.volume | 19 | en_US |
mus.contributor.orcid | Stewart, Philip S.|0000-0001-7773-8570 | en_US |
mus.data.thumbpage | 3 | en_US |
mus.identifier.category | Chemical & Material Sciences | en_US |
mus.identifier.category | Engineering & Computer Science | en_US |
mus.identifier.category | Health & Medical Sciences | en_US |
mus.identifier.category | Life Sciences & Earth Sciences | en_US |
mus.identifier.doi | 10.12968/jowc.2010.19.6.48468 | en_US |
mus.relation.college | College of Engineering | en_US |
mus.relation.department | Center for Biofilm Engineering. | en_US |
mus.relation.department | Chemical & Biological Engineering. | en_US |
mus.relation.researchgroup | Center for Biofilm Engineering. | en_US |
mus.relation.university | Montana State University - Bozeman | en_US |
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