Testing wound dressings using an in vitro wound model

dc.contributor.authorLipp, C.
dc.contributor.authorKirker, Kelly R.
dc.contributor.authorAgostinho, Alessandra
dc.contributor.authorJames, Garth A.
dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-04-11T19:40:49Z
dc.date.available2017-04-11T19:40:49Z
dc.date.issued2010-06
dc.description.abstractObjective: To determine whether or not there are any significant differences in the effects of wound dressings on bacterial bioburden. Method: A selection of non-occlusive, non-adhesive dressings was tested for their effect on bacterial bioburden. The dressings selected included two dressings with antimicrobial properties (one containing silver and one containing PHMB), a cotton-based dressing enclosed in a perforated sleeve of poly(ethylene terephthalate), a carboxymethyl cellulose-based dressing, a fiber-free alginate dressing, and a 12-ply 100% cotton gauze. Using the colony-drip flow reactor (DFR) model, a meticillin-resistant Staphylococcus aureus (MRSA) or Pseudomonas aeruginosa biofilm was grown underneath a dressing sample. Biofilm growth was examined via plate counts, fluorescent microscopy and scanning electron microscopy. Results: The dressings containing antimicrobial agents had the greatest effect on bacterial load. In the MRSA experiments, both antimicrobial dressings produced lower bacteria counts than the other dressings (p<0.001), while in the P. aeruginosa experiments, only the silver-containing sample had fewer bacteria (p<0.0001). However, neither antimicrobial dressing was able to completely eradicate the bacteria when testing with either microorganism. Conclusion: The results presented herein illustrate that bacteria can grow unchallenged within the dressing environment and that an antimicrobial dressing can limit this bacterial growth. Declaration of interest: None.en_US
dc.identifier.citationLipp C, Kirker K, Agostinho A, James G, Stewart PS, "Testing wound dressings using an in vitro wound model," J Wound Care, 2010 19(6):220-226.en_US
dc.identifier.issn0969-0700
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/12694
dc.titleTesting wound dressings using an in vitro wound modelen_US
dc.typeArticleen_US
mus.citation.extentfirstpage220en_US
mus.citation.extentlastpage226en_US
mus.citation.issue6en_US
mus.citation.journaltitleJournal of Wound Careen_US
mus.citation.volume19en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US
mus.data.thumbpage3en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.12968/jowc.2010.19.6.48468en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
00-022_Dissimilatory_Iron-reducing_bacteria.pdf
Size:
403.68 KB
Format:
Adobe Portable Document Format
Description:
Testing wound dressings using an in vitro wound model (PDF)
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
826 B
Format:
Item-specific license agreed upon to submission
Description:
Copyright (c) 2002-2022, LYRASIS. All rights reserved.