Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing

Abstract

Purpose: To develop an interproximal laboratory model to compare the potential effectiveness of powered brushing to remove biofilm plaque from interproximal spaces beyond the reach of bristles. Materials and Methods: Streptococcus mutans biofilms were first grown onglass microscope slides in a drip-flow reactor. The slides were removed and positioned in the interproximal model. Each slide was exposed to 15 seconds powered brushing with either the Sonicare® Elite or the Braun Oral-B 3D Excel. The thickness of the biofilm was measured with confocal microscopy at various distances from the bristle tips. Results: The Sonicare® Elite reduced the thickness of biofilm by 57% at a distance of 0-5 mm from the bristle tips, 46% at 5-10 mm and 43% at 10-15 mm, relative to biofilm in areas unexposed to brushing. All reductions in thickness were statistically significant (P< 0.01). The Braun Oral-B 3D reduced the biofilm thickness by 16%, 13%, and 19% at the same distances respectively, but the thickness reductions were not statistically significant from those in the unexposed areas (P> 0.1).