Viable microbes in ice: Application of molecular assays to McMurdo Dry Valley lake ice communities

dc.contributor.authorDieser, Markus
dc.contributor.authorNocker, Andreas
dc.contributor.authorPriscu, John C.
dc.contributor.authorForeman, Christine M.
dc.date.accessioned2017-06-21T14:53:44Z
dc.date.available2017-06-21T14:53:44Z
dc.date.issued2010-06
dc.description.abstractThe permanent ice covers of the McMurdo Dry Valley lakes, Antarctica, are colonized by a diverse microbial assemblage. We collected ice cores from Lakes Fryxell, Hoare and Bonney. Propidium monoazide (PMA) was used in combination with quantitative PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE) to examine membrane integrity of prokaryotes in these extreme environments. PMA selectively penetrates cells with compromised membranes and modifies their DNA resulting in the suppression of PCR amplification. Our results based on analysis of 16S rRNA genes demonstrate that despite the hostile conditions of the Dry Valleys, the permanent ice covers of the lakes support a ‘potentially viable’ microbial community. The level of membrane integrity, as well as diversity, was higher in samples where sediment was entrapped in the ice cover. Pronounced differences in the fraction of cells with intact and compromised cell membranes were found for Lake Fryxell and east lobe of Lake Bonney, both expressed in differences in DGGE banding patterns and qPCR signal reductions. Limitations in the ability to distinguish between intact or compromised cells occurred in samples from Lake Hoare and west lobe of Lake Bonney due to low DNA template concentrations recovered from the samples.en_US
dc.identifier.citationDieser M, Nocker A, Priscu JC, Foreman CM, "Viable microbes in ice: Application of molecular assays to McMurdo Dry Valley lake ice communities," Antarctic Science 2010 22(5):470-476en_US
dc.identifier.issn0954-1020
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/13115
dc.titleViable microbes in ice: Application of molecular assays to McMurdo Dry Valley lake ice communitiesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage470en_US
mus.citation.extentlastpage476en_US
mus.citation.issue5en_US
mus.citation.journaltitleAntarctic Scienceen_US
mus.citation.volume22en_US
mus.contributor.orcidForeman, Christine M.|0000-0003-0230-4692en_US
mus.data.thumbpage3en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1017/s0954102010000404en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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