Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity
dc.contributor.author | Hystad, Steven M. | |
dc.contributor.author | Martin, John M. | |
dc.contributor.author | Graybosch, R.A. | |
dc.contributor.author | Giroux, Michael J. | |
dc.date.accessioned | 2016-02-16T23:27:13Z | |
dc.date.available | 2016-02-16T23:27:13Z | |
dc.date.issued | 2015-08 | |
dc.description.abstract | Wheat (Triticum aestivum) polyphenol oxidase (PPO) contributes to the time-dependent discoloration of Asian noodles. Wheat contains multiple paralogous and orthologous Ppo genes, Ppo-A1, Ppo-D1, Ppo-A2, Ppo-D2, and Ppo-B2, expressed in wheat kernels. To date, wheat noodle color improvement efforts have focused on breeding cultivars containing Ppo-D1 and Ppo-A1 alleles conferring reduced PPO activity. A major impediment to wheat quality improvement is a lack of additional Ppo alleles conferring reduced kernel PPO. In this study, a previously reported very low PPO line, 07OR1074, was found to contain a novel allele at Ppo-A2 and null alleles at the Ppo-A1 and Ppo-D1 loci. To examine the impact of each mutation upon kernel PPO, populations were generated from crosses between 07OR1074 and the hard white spring wheat cultivars Choteau and Vida. Expression analysis using RNA-seq demonstrated no detectable Ppo-A1 transcripts in 07OR1074 while Ppo-D1 transcripts were present at less than 10 % of that seen in Choteau and Vida. Novel markers specific for the Ppo-D1 and Ppo-A2 mutations discovered in 07OR1074, along with the Ppo-A1 STS marker, were used to screen segregating populations. Evaluation of lines indicated a substantial genotypic effect on PPO with Ppo-A1 and Ppo-D1 alleles contributing significantly to total PPO in both populations. These results show that the novel mutations in Ppo-A1 and Ppo-D1 present in 07OR1074 are both important to lowering overall wheat seed PPO activity and may be useful to produce more desirable and marketable wheat-based products. | en_US |
dc.description.sponsorship | Montana Wheat and Barley Committee | en_US |
dc.identifier.citation | Hystad, S. M., J. M. Martin, R. A. Graybosch, and M. J. Giroux. "Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity.." Theoretical & Applied Genetics 128, no. 8 (August 2015): 1605-1615. https://dx.doi.org/10.1007/s00122-015-2535-8. | en_US |
dc.identifier.issn | 0040-5752 | |
dc.identifier.uri | https://scholarworks.montana.edu/handle/1/9574 | |
dc.title | Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity | en_US |
dc.type | Article | en_US |
mus.citation.extentfirstpage | 1605 | en_US |
mus.citation.extentlastpage | 1615 | en_US |
mus.citation.issue | 8 | en_US |
mus.citation.journaltitle | Theoretical & Applied Genetics | en_US |
mus.citation.volume | 128 | en_US |
mus.data.thumbpage | 3 | en_US |
mus.identifier.category | Life Sciences & Earth Sciences | en_US |
mus.identifier.doi | 10.1007/s00122-015-2535-8 | en_US |
mus.relation.college | College of Agriculture | en_US |
mus.relation.department | Plant Sciences & Plant Pathology. | en_US |
mus.relation.university | Montana State University - Bozeman | en_US |
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