Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity

dc.contributor.authorHystad, Steven M.
dc.contributor.authorMartin, John M.
dc.contributor.authorGraybosch, R.A.
dc.contributor.authorGiroux, Michael J.
dc.date.accessioned2016-02-16T23:27:13Z
dc.date.available2016-02-16T23:27:13Z
dc.date.issued2015-08
dc.description.abstractWheat (Triticum aestivum) polyphenol oxidase (PPO) contributes to the time-dependent discoloration of Asian noodles. Wheat contains multiple paralogous and orthologous Ppo genes, Ppo-A1, Ppo-D1, Ppo-A2, Ppo-D2, and Ppo-B2, expressed in wheat kernels. To date, wheat noodle color improvement efforts have focused on breeding cultivars containing Ppo-D1 and Ppo-A1 alleles conferring reduced PPO activity. A major impediment to wheat quality improvement is a lack of additional Ppo alleles conferring reduced kernel PPO. In this study, a previously reported very low PPO line, 07OR1074, was found to contain a novel allele at Ppo-A2 and null alleles at the Ppo-A1 and Ppo-D1 loci. To examine the impact of each mutation upon kernel PPO, populations were generated from crosses between 07OR1074 and the hard white spring wheat cultivars Choteau and Vida. Expression analysis using RNA-seq demonstrated no detectable Ppo-A1 transcripts in 07OR1074 while Ppo-D1 transcripts were present at less than 10 % of that seen in Choteau and Vida. Novel markers specific for the Ppo-D1 and Ppo-A2 mutations discovered in 07OR1074, along with the Ppo-A1 STS marker, were used to screen segregating populations. Evaluation of lines indicated a substantial genotypic effect on PPO with Ppo-A1 and Ppo-D1 alleles contributing significantly to total PPO in both populations. These results show that the novel mutations in Ppo-A1 and Ppo-D1 present in 07OR1074 are both important to lowering overall wheat seed PPO activity and may be useful to produce more desirable and marketable wheat-based products.en_US
dc.description.sponsorshipMontana Wheat and Barley Committeeen_US
dc.identifier.citationHystad, S. M., J. M. Martin, R. A. Graybosch, and M. J. Giroux. "Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity.." Theoretical & Applied Genetics 128, no. 8 (August 2015): 1605-1615. https://dx.doi.org/10.1007/s00122-015-2535-8.en_US
dc.identifier.issn0040-5752
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/9574
dc.titleGenetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activityen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1605en_US
mus.citation.extentlastpage1615en_US
mus.citation.issue8en_US
mus.citation.journaltitleTheoretical & Applied Geneticsen_US
mus.citation.volume128en_US
mus.data.thumbpage3en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1007/s00122-015-2535-8en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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