Browsing by Author "Langille, S. E."

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Adhesion of biofilms to inert surfaces: a molecular level approach directed at the marine environments
(1996-09) Baty, Ace M.; Frolund, B.; Geesey, Gill G.; Langille, S. E.; Quintero, Ernesto J.; Suci, Peter A.; Weiner, R. M.
Protein/ligand interactions involved in mediating adhesion between microorganisms and biological surfaces have been well‐characterized in some cases (e.g. pathogen/host interactions). The strategies microorganisms employ for attachment to inert surfaces have not been so clearly elucidated. An experimental approach is presented which addresses the issues from the point of view of molecular interactions occurring at the interface.
Function of bacterial (hyphomonas spp.) capsular exopolymers in biofouling
(1997) Weiner, R. M.; Langille, S. E.; Geesey, Gill G.; Quintero, Ernesto J.
Influence of protein conditioning films on binding of a bacterial polysaccharide adhesin from hyphomonas mhs-3
(1996-09) Frolund, B.; Suci, Peter A.; Langille, S. E.; Weiner, R. M.; Geesey, Gill G.
A putative polysaccharide adhesin which mediates non‐specific attachment of Hyphomonas MHS‐3 (MHS‐3) to hydrophilic substrata has been isolated and partially characterized. A polysaccharide‐enriched portion of the extracellular polymeric substance (EPSP) from MHS‐3 was separated into four fractions using high performance size exclusion chromatography (HPSEC). Comparison of chromatograms of EPSP from MHS‐3 and a reduced adhesion strain (MHS‐3 rad) suggested that one EPSP fraction, which consisted of carbohydrate, served as an adhesin. Adsorption of this fraction to germanium (Ge) was investigated using attenuated total reflection Fourier transform infrared (ATR/FT‐IR) spectrometry. Binding curves indicated that the isolated fraction had a relatively high affinity for Ge when ranked against an adhesive protein from Mytilis edulis, mussel adhesive protein (MAP) and an acidic polysaccharide (alginate from Macrocystis pyrifera). Spectral features were used to identify the fraction as a polysaccharide previously reported to adsorb preferentially out of the EPSP mixture. Conditioning the Ge substratum with either bovine serum albumin (BSA) or MAP decreased the adsorption of the adhesive polysaccharide significantly. Conditioning Ge with these proteins also decreased adhesion of whole cells.
Polysaccharide-specific probes inhibit adhesion of hyphomonas rosenbergii strain vp-6 to hydrophilic surfaces
(2000-08) Langille, S. E.; Geesey, Gill G.; Weiner, R. M.
Biofilm formation commences with the adhesion of microorganisms to surfaces. Information regarding the initial bond between a bacterium and a solid surface is essential for devising methods to inhibit the onset of biofilm formation. Three different types of polysaccharide-specific probes, cationic metals, dyes, and lectins, were used to bind the exopolysaccharide of Hyphomonas rosenbergii, a budding, prosthecate marine bacterium. Probes, which specifically bind complex carbohydrates, inhibit the adhesion of H. rosenbergii to hydrophilic surfaces. These results suggest that the polysaccharide portion of H. rosenbergii capsular, extracellular polymeric-substance is involved in the primary adhesion process. Journal of Industrial Microbiology & Biotechnology (2000) 25, 81–85.