Scholarly Work - Center for Biofilm Engineering
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/9335
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Item Fate of cyanide in aerated microbial systems(1975) Raef, S. F.; Characklis, William G.; Kessick, M. A.Item Fate of cyanide and related compounds in industrial waste treatment(1974) Raef, S. F.; Characklis, William G.Item Fate of cyanide and related compounds in aerobic microbial systems-I. Chemical reaction with substrate and physical removal(1977) Raef, S. F.; Characklis, William G.; Kessick, M. A.; Ward, C. H.The chemical reaction of cyanide with substrate was investigated in sealed glass ampoules using glucose as substrate and inorganic buffers. The reaction was found to be pseudo-first order and pH dependent, with an optimum pH near 11.0. The cyanide-glucose reaction products were found to be biodegradable by both acclimated and unacclimated heterogeneous cultures in shake flask and BOD bottle systems.Adsorption onto microbial solids was investigated using sealed, stirred glass reactors containing bacteria and potassium cyanide in water buffered at pH 7.0 with inorganic buffers. Very little adsorption occurred on a starved non-flocculating pure culture of Bacillus meqaterium, although up to 15% adsorption occurred in systems containing a stirred flocculent heterogeneous culture.Stripping was investigated from a starved heterogeneous culture in an aerated microfermenter at neutral pH. Hydrogen cyanide and carbon dioxide in the off-gas were trapped in sodium hydroxide solution, separated and analyzed. Stripping removed up to 80% of original cyanide, and tests using K14CN revealed that a small amount of cyanide had been metabolized.Item Fate of cyanide and related compounds in aerobic microbial systems-II. Microbial degradation(1977) Raef, S. F.; Characklis, William G.; Kessick, M. A.; Ward, C. H.Cyanide metabolism was studied using starved, acclimated heterogeneous cultures in an aerated microfermenter containing glucose as substrate. Tests using K14 CN indicated that up to 50% of the cyanide was metabolized as evidenced by 14CO2 production. Experiments employed initial solids concentrations between 483-1963 mgl and initial glucose concentrations between 100-600 mg l. Initial cyanide concentration was 10 mg l.