Center for Biofilm Engineering (CBE)
Permanent URI for this communityhttps://scholarworks.montana.edu/handle/1/9334
At the Center for Biofilm Engineering (CBE), multidisciplinary research teams develop beneficial uses for microbial biofilms and find solutions to industrially relevant biofilm problems. The CBE was established at Montana State University, Bozeman, in 1990 as a National Science Foundation Engineering Research Center. As part of the MSU College of Engineering, the CBE gives students a chance to get a head start on their careers by working on research teams led by world-recognized leaders in the biofilm field.
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Item Change Rippling through Our Waters and Culture(Wiley, 2020-04) Martin, Christine; Doyle, John; LaFrance, JoRee; Lefthand, Myra J.; Young, Sara L.; Three Irons, Emery; Eggers, Margaret J.It is well established that climate change is already causing a wide variety of human health impacts in the United States and globally, and that for many reasons Native Americans are particularly vulnerable. Tribal water security is particularly threatened; the ways in which climate changes are damaging community health and well-being through impacts on water resources have been addressed more thoroughly for Tribes in coastal, arid, and sub-arctic/arctic regions of the United States. In this article, Crow Tribal members from the Northern Plains describe the impacts of climate and environmental change on local water resources and ecosystems, and thereby on Tribal community health and well-being. Formal, qualitative research methodology was employed drawing on interviews with 26 Crow Tribal Elders. Multiple determinants of health are addressed, including cultural, social, economic, and environmental factors. The sense of environmental-cultural-health loss and despair at the inability to address the root causes of climate change are widespread. Yet the co-authors and many other Tribal members are actively prioritizing, addressing, and coping with some of the local impacts of these changes, and are carrying on Apsáalooke [Crow] lifeways and values.Item Change Rippling through Our Waters and Culture(Wiley, 2020-04) Martin, Christine; Doyle, John; LaFrance, JoRee; Lefthand, Myra J.; Young, Sara L.; Three Irons, Emery; Eggers, Margaret J.It is well established that climate change is already causing a wide variety of human health impacts in the United States and globally, and that for many reasons Native Americans are particularly vulnerable. Tribal water security is particularly threatened; the ways in which climate changes are damaging community health and well-being through impacts on water resources have been addressed more thoroughly for Tribes in coastal, arid, and sub-arctic/arctic regions of the United States. In this article, Crow Tribal members from the Northern Plains describe the impacts of climate and environmental change on local water resources and ecosystems, and thereby on Tribal community health and well-being. Formal, qualitative research methodology was employed drawing on interviews with 26 Crow Tribal Elders. Multiple determinants of health are addressed, including cultural, social, economic, and environmental factors. The sense of environmental-cultural-health loss and despair at the inability to address the root causes of climate change are widespread. Yet the co-authors and many other Tribal members are actively prioritizing, addressing, and coping with some of the local impacts of these changes, and are carrying on Apsáalooke [Crow] lifeways and values.Item Contribution of wild foods to diet, food security, and cultural values amidst climate change(2019-11) Smith, Erin; Ahmed, Selena; Running Crane, MaryAnn; Eggers, Margaret J.; Pierre, Mike; Flagg, Kenneth A.; Byker Shanks, CarmenWild foods are recognized to contribute to diet and food security through enhancing the availability of local, diverse, and nonmarket food sources. We investigated the contribution of wild foods to diet, food security, and cultural identity in a Native American[1] community in the context of climate change. Structured interviews were conducted with low-income residents of the Flathead Indian Reservation[2] in Northwestern Montana who participate in the federal Food Distribution Program on Indian Reservations, also known by participants as ‘Commodities.’ Responses to structured questions were analyzed for frequency, and open-ended responses were coded and analyzed to identify prevalent themes. Our analysis indicated that half of participants were food insecure. Approximately 28% of participants engaged in at least one wild food procurement activity, including hunting, fishing, and harvesting. On average, participants who engaged in one or more wild food procurement activities were more food secure than those who did not. Results highlight the multidimensional valuation of wild foods by participants including taste, freshness, nutritional quality, being a traditional community practice, and providing a sense of self-sufficiency. Climate change is perceived by participants to be adversely impacting wild food systems due to increased variability in seasonality and precipitation and increased incidences of wild fire. Findings point to the need for community-based strategies to strengthen wild food knowledge toward enhancing food sovereignty in Native American communities, in the context of climate change. [1] The term ‘Native American’ was determined to be the preferred term for referencing the Native American community in this study, based on consultation from our community advisory board. [2] The term ‘Flathead Indian Reservation’ was determined to be the preferred term for referencing the location in which this study was held, based on consultation from our community advisory board.Item Metagenomic Profiling of Microbial Pathogens in the Little Bighorn River, Montana(2019-03) Hamner, Steve; Brown, Bonnie L.; Hasan, Nur A.; Franklin, Michael J.; Doyle, John T.; Eggers, Margaret J.; Colwell, Rita R.; Ford, Tim E.The Little Bighorn River is the primary source of water for water treatment plants serving the local Crow Agency population, and has special significance in the spiritual and ceremonial life of the Crow tribe. Unfortunately, the watershed suffers from impaired water quality, with high counts of fecal coliform bacteria routinely measured during run-off events. A metagenomic analysis was carried out to identify potential pathogens in the river water. The Oxford Nanopore MinION platform was used to sequence DNA in near real time to identify both uncultured and a coliform-enriched culture of microbes collected from a popular summer swimming area of the Little Bighorn River. Sequences were analyzed using CosmosID bioinformatics and, in agreement with previous studies, enterohemorrhagic and enteropathogenic Escherichia coli and other E. coli pathotypes were identified. Noteworthy was detection and identification of enteroaggregative E. coli O104:H4 and Vibrio cholerae serotype O1 El Tor, however, cholera toxin genes were not identified. Other pathogenic microbes, as well as virulence genes and antimicrobial resistance markers, were also identified and characterized by metagenomic analyses. It is concluded that metagenomics provides a useful and potentially routine tool for identifying in an in-depth manner microbial contamination of waterways and, thereby, protecting public health.Item Detection of Pathogenic and Non-pathogenic Bacteria in Drinking Water and Associated Biofilms on the Crow Reservation, Montana, USA(2018-07) Richards, Crystal L.; Broadaway, Susan C.; Eggers, Margaret J.; Doyle, John T.; Pyle, Barry H.; Camper, Anne K.; Ford, Tim E.Private residences in rural areas with water systems that are not adequately regulated, monitored, and updated could have drinking water that poses a health risk. To investigate water quality on the Crow Reservation in Montana, water and biofilm samples were collected from 57 public buildings and private residences served by either treated municipal or individual groundwater well systems. Bacteriological quality was assessed including detection of fecal coliform bacteria and heterotrophic plate count (HPC) as well as three potentially pathogenic bacterial genera, Mycobacterium, Legionella, and Helicobacter. All three target genera were detected in drinking water systems on the Crow Reservation. Species detected included the opportunistic and frank pathogens Mycobacterium avium, Mycobacterium gordonae, Mycobacterium flavescens, Legionella pneumophila, and Helicobacter pylori. Additionally, there was an association between HPC bacteria and the presence of Mycobacterium and Legionella but not the presence of Helicobacter. This research has shown that groundwater and municipal drinking water systems on the Crow Reservation can harbor potential bacterial pathogens.Item Challenges and Opportunities for Tribal Waters: Addressing Disparities in Safe Public Drinking Water on the Crow Reservation in Montana, US(2018-03) Doyle, John T.; Kindness, L.; Real Bird, James; Eggers, Margaret J.; Camper, Anne K.Disparities in access to safe public drinking water are increasingly being recognized as contributing to health disparities and environmental injustice for vulnerable communities in the United States. As the Co-Directors of the Apsaálooke Water and Wastewater Authority (AWWWA) for the Crow Tribe, with our academic partners, we present here the multiple and complex challenges we have addressed in improving and maintaining tribal water and wastewater infrastructure, including the identification of diverse funding sources for infrastructure construction, the need for many kinds of specialized expertise and long-term stability of project personnel, ratepayer difficulty in paying for services, an ongoing legacy of inadequate infrastructure planning, and lack of water quality research capacity. As a tribal entity, the AWWWA faces additional challenges, including the complex jurisdictional issues affecting all phases of our work, lack of authority to create water districts, and additional legal and regulatory gaps—especially with regards to environmental protection. Despite these obstacles, the AWWWA and Crow Tribe have successfully upgraded much of the local water and wastewater infrastructure. We find that ensuring safe public drinking water for tribal and other disadvantaged U.S. communities will require comprehensive, community-engaged approaches across a broad range of stakeholders to successfully address these complex legal, regulatory, policy, community capacity, and financial challenges.Item Community Engaged Cumulative Risk Assessment of Exposure to Inorganic Well Water Contaminants, Crow Reservation, Montana(2018-01) Eggers, Margaret J.; Doyle, John T.; Lefthand, M. J.; Young, Sara L.; Moore-Nall, Anita L.; Kindness, L.; Medicine, R. O.; Ford, Tim E.; Dietrich, E.; Parker, Albert E.; Hoover, J. H.; Camper, Anne K.An estimated 11 million people in the US have home wells with unsafe levels of hazardous metals and nitrate. The national scope of the health risk from consuming this water has not been assessed as home wells are largely unregulated and data on well water treatment and consumption are lacking. Here, we assessed health risks from consumption of contaminated well water on the Crow Reservation by conducting a community-engaged, cumulative risk assessment. Well water testing, surveys and interviews were used to collect data on contaminant concentrations, water treatment methods, well water consumption, and well and septic system protection and maintenance practices. Additive Hazard Index calculations show that the water in more than 39% of wells is unsafe due to uranium, manganese, nitrate, zinc and/or arsenic. Most families’ financial resources are limited, and 95% of participants do not employ water treatment technologies. Despite widespread high total dissolved solids, poor taste and odor, 80% of families consume their well water. Lack of environmental health literacy about well water safety, pre-existing health conditions and limited environmental enforcement also contribute to vulnerability. Ensuring access to safe drinking water and providing accompanying education are urgent public health priorities for Crow and other rural US families with low environmental health literacy and limited financial resources.Item Community-based participatory research in Indian country: Improving health through water quality research and awareness(2010-07) Cummins, C.; Doyle, John T.; Kindness, L.; Lefthand, M. J.; Bear Don't Walk, U. J.; Bends, Ada L.; Broadaway, Susan C.; Camper, Anne K.; Fitch, R.; Ford, Tim E.; Hamner, Steve; Morrison, A. R.; Richards, Crystal L.; Young, Sara L.; Eggers, Margaret J.Water has always been held in high respect by the Apsaalooke (Crow) people of Montana. Tribal members questioned the health of the rivers and well water because of visible water quality deterioration and potential connections to illnesses in the community. Community members initiated collaboration among local organizations, the tribe, and academic partners, resulting in genuine community-based participatory research. The article shares what we have learned as tribal members and researchers about working together to examine surface and groundwater contaminants, assess routes of exposure, and use our data to bring about improved health of our people and our waters.Item Applying indigenous CBPR principles to partnership development in health disparities research(2011-07) Christopher, S.; Saha, R.; Lachapelle, Paul; Jennings, D.; Colclough, Y.; Cooper, C.; Cummins, C.; Eggers, Margaret J.; FourStar, Kris; Harris, K.; Kuntz, Sandra W.; LaFromboise, V.; LaVeaux, Deb; McDonald, T.; Real Bird, James; Rink, Elizabeth; Webster, C.This case study of community and university research partnerships utilizes previously developed principles for conducting research in the context of Native American communities to consider how partners understand and apply the principles in developing community-based participatory research partnerships to reduce health disparities. The 7 partnership projects are coordinated through a National Institutes of Health–funded center and involve a variety of tribal members, including both health care professionals and lay persons and native and nonnative university researchers. This article provides detailed examples of how these principles are applied to the projects and discusses the overarching and interrelated emergent themes of sharing power and building trust.Item Exploring effects of climate change on Northern Plains American Indian health(2013-06) Doyle, John T.; Redsteer, M. H.; Eggers, Margaret J.American Indians have unique vulnerabilities to the impacts of climate change because of the links among ecosystems, cultural practices, and public health, but also as a result of limited resources available to address infrastructure needs. On the Crow Reservation in south-central Montana, a Northern Plains American Indian Reservation, there are community concerns about the consequences of climate change impacts for community health and local ecosystems. Observations made by Tribal Elders about decreasing annual snowfall and milder winter temperatures over the 20th century initiated an investigation of local climate and hydrologic data by the Tribal College. The resulting analysis of meteorological data confirmed the decline in annual snowfall and an increase in frost free days. In addition, the data show a shift in precipitation from winter to early spring. The number of days exceeding 90 °F (32 °C) has doubled in the past century. Streamflow data show a long-term trend of declining discharge. Elders noted that the changes are affecting fish distribution within local streams and plant species which provide subsistence foods. Concerns about warmer summer temperatures also include heat exposure during outdoor ceremonies that involve days of fasting without food or water. Additional community concerns about the effects of climate change include increasing flood frequency and fire severity, as well as declining water quality. The authors call for local research to understand and document current effects and project future impacts as a basis for planning adaptive strategies.