University Information Technology

Permanent URI for this communityhttps://scholarworks.montana.edu/handle/1/10011

In support of the Montana State University mission, University Information Technology promotes, develops, delivers, and facilitates the use of information technology services and resources. We were formerly called Information Technology Center. University Information Technology

Browse

Filters

20141

Settings

search.filters.applied.f.department: search.filters.department.Genetics.

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    In situ gene expression profiling of the thermoacidophilic alga C yanidioschyzon in relation to visible and ultraviolet irradiance
    (2014-06) Skorupa, Dana J.; Castenholz, R. W.; Mazurie, Aurélien J.; Carey, Charles C.; Rosenzweig, F.; McDermott, Timothy R.
    Ultraviolet and high-intensity visible radiation generate reactive intermediates that damage phototrophic microorganisms. In Yellowstone National Park, the thermoacidophilic alga Cyanidioschyzon exhibits an annual seasonal biomass fluctuation referred to as 'mat decline', where algal viability decreases as ultraviolet and visible irradiances increase during summer. We examined the role irradiance might play in mat decline using irradiance filters that uncouple ultraviolet and visible effects along with custom microarrays to study gene expression in situ. Of the 6,507 genes, 88% showed no response to ultraviolet or visible, implying that at the biomolecular level, these algae inhabit a chemostat-like environment and is consistent with the near constant aqueous chemistry measured. The remaining genes exhibited expression changes linked to ultraviolet exposure, to increased visible radiation, or to the apparent combined effects of ultraviolet and visible. Expression of DNA repetitive elements was synchronized, being repressed by visible but also influenced by ultraviolet. At highest irradiance levels, these algae reduced transcription of genes encoding functions involved with DNA replication, photosynthesis and cell cycle progression but exhibited an uptick in activities related to repairing DNA damage. This corroborates known physiological responses to ultraviolet and visible radiation, and leads us to provisionally conclude that mat decline is linked to photoinhibition.
Copyright (c) 2002-2022, LYRASIS. All rights reserved.